Clonal dissemination and colony morphotype variation of vancomycin-resistant Enterococcus faecium isolates in metropolitan Detroit, Michigan - PubMed (original) (raw)

Clonal dissemination and colony morphotype variation of vancomycin-resistant Enterococcus faecium isolates in metropolitan Detroit, Michigan

W M Dunne Jr et al. J Clin Microbiol. 1997 Feb.

Abstract

Thirty-two isolates of vancomycin-resistant Enterococcus faecium (VRE) recovered from 25 patients hospitalized at six hospitals in the metropolitan Detroit, Mich., area over a 32-month period were examined for relatedness by repetitive-sequence PCR (rep-PCR). All isolates were shown to carry the vanA gene by PCR. The rep-PCR patterns generated from each isolate showed that the first three VRE isolates obtained from hospital A between June 1992 and February 1994 were distinct strains. Thereafter, all VRE isolates originating from hospital A and those collected from five other area hospitals had identical rep-PCR patterns. On detailed examination, subcultures of 25 of the 32 VRE isolates produced two distinct colony types characterized phenotypically by a rough and a smooth appearance, respectively. Both colony types retained the vanA locus and the rep-PCR pattern of the primary isolate. These data suggest that a single strain of VRE with the capacity to produce two colonial variants has been disseminated to several Detroit-area hospitals. The clinical significance of the colonial morphotypes is unclear.

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References

    1. Clin Infect Dis. 1995 Apr;20(4):836-41 -PubMed
    1. J Clin Microbiol. 1990 Sep;28(9):2059-63 -PubMed
    1. J Clin Microbiol. 1995 Nov;33(11):2842-6 -PubMed
    1. Clin Infect Dis. 1995 Nov;21(5):1234-7 -PubMed
    1. Br J Biomed Sci. 1995 Dec;52(4):321-2 -PubMed

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