Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G - PubMed (original) (raw)
. 1996 Dec 16;15(24):7168-77.
Affiliations
- PMID: 9003792
- PMCID: PMC452544
Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G
S Z Tarun Jr et al. EMBO J. 1996.
Abstract
Although the cap structure and the poly(A) tail are on opposite ends of the mRNA molecule, previous work has suggested that they interact to enhance translation and inhibit mRNA degradation. Here we present biochemical data that show that the proteins bound to the mRNA cap (eIF-4F) and poly(A) tail (Pab1p) are physically associated in extracts from the yeast Saccharomyces cerevisiae. Specifically, we find that Pab1p co-purifies and co-immunoprecipitates with the eIF-4G subunit of eIF-4F. The Pab1p binding site on the recombinant yeast eIF-4G protein Tif4632p was mapped to a 114-amino-acid region just proximal to its eIF-4E binding site. Pab1p only bound to this region when complexed to poly(A). These data support the model that the Pablp-poly(A) tail complex on mRNA can interact with the cap structure via eIF-4G.
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References
- EMBO J. 1996 Mar 15;15(6):1371-82 - PubMed
- EMBO J. 1995 Dec 15;14(24):6301-10 - PubMed
- Mol Cell Biol. 1987 Sep;7(9):3268-76 - PubMed
- Mol Cell Biol. 1988 Aug;8(8):3556-9 - PubMed
- Gene. 1988 Oct 30;70(2):303-12 - PubMed
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