GABA(B)-mediated presynaptic inhibition of excitatory transmission and synaptic vesicle dynamics in cultured hippocampal neurons - PubMed (original) (raw)
GABA(B)-mediated presynaptic inhibition of excitatory transmission and synaptic vesicle dynamics in cultured hippocampal neurons
J S Isaacson et al. Neuron. 1997 Jan.
Free article
Abstract
Local recycling of synaptic vesicle membrane at nerve terminals is necessary to maintain a readily releasable pool of transmitter. To what extent are the dynamics of vesicle recycling subject to modulation? We examined the influence of presynaptic GABA(B) receptors on vesicle dynamics at single synapses using optical imaging of FM1-43 in cultured rat hippocampal neurons. The kinetics of FM1-43 destaining indicate that synapses from a single neuron have a unimodal distribution of release probabilities, and GABA(B)-mediated inhibition occurs uniformly at all sites. Electrical and optical recordings from single cells show that the inhibition of excitatory transmission is entirely accounted for by a rapidly reversible reduction of exocytosis. In contrast, GABA(B) receptors do not alter the rate or extent of endocytosis.
Similar articles
- Physiological activity depresses synaptic function through an effect on vesicle priming.
Moulder KL, Jiang X, Taylor AA, Olney JW, Mennerick S. Moulder KL, et al. J Neurosci. 2006 Jun 14;26(24):6618-26. doi: 10.1523/JNEUROSCI.5498-05.2006. J Neurosci. 2006. PMID: 16775150 Free PMC article. - Potassium ion- and nitric oxide-induced exocytosis from populations of hippocampal synapses during synaptic maturation in vitro.
Sporns O, Jenkinson S. Sporns O, et al. Neuroscience. 1997 Oct;80(4):1057-73. doi: 10.1016/s0306-4522(97)00152-8. Neuroscience. 1997. PMID: 9284060 - Depression of glutamatergic and GABAergic synaptic responses in striatal spiny neurons by stimulation of presynaptic GABAB receptors.
Nisenbaum ES, Berger TW, Grace AA. Nisenbaum ES, et al. Synapse. 1993 Jul;14(3):221-42. doi: 10.1002/syn.890140306. Synapse. 1993. PMID: 8105549 - GABA vesicles at synapses: are there 2 distinct pools?
Hablitz JJ, Mathew SS, Pozzo-Miller L. Hablitz JJ, et al. Neuroscientist. 2009 Jun;15(3):218-24. doi: 10.1177/1073858408326431. Neuroscientist. 2009. PMID: 19436074 Free PMC article. Review. - Single vesicle tracking for studying synaptic vesicle dynamics in small central synapses.
Park C, Jung S, Park H. Park C, et al. Curr Opin Neurobiol. 2022 Oct;76:102596. doi: 10.1016/j.conb.2022.102596. Epub 2022 Jul 6. Curr Opin Neurobiol. 2022. PMID: 35803103 Review.
Cited by
- FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region.
Yamamoto Y, Kida H, Kagawa Y, Yasumoto Y, Miyazaki H, Islam A, Ogata M, Yanagawa Y, Mitsushima D, Fukunaga K, Owada Y. Yamamoto Y, et al. J Neurosci. 2018 Dec 5;38(49):10411-10423. doi: 10.1523/JNEUROSCI.1285-18.2018. Epub 2018 Oct 19. J Neurosci. 2018. PMID: 30341178 Free PMC article. - Dopamine presynaptically and heterogeneously modulates nucleus accumbens medium-spiny neuron GABA synapses in vitro.
Geldwert D, Norris JM, Feldman IG, Schulman JJ, Joyce MP, Rayport S. Geldwert D, et al. BMC Neurosci. 2006 Jun 30;7:53. doi: 10.1186/1471-2202-7-53. BMC Neurosci. 2006. PMID: 16813648 Free PMC article. - Regulation of exocytosis from single visualized GABAergic boutons in hippocampal slices.
Brager DH, Luther PW, Erdélyi F, Szabó G, Alger BE. Brager DH, et al. J Neurosci. 2003 Nov 19;23(33):10475-86. doi: 10.1523/JNEUROSCI.23-33-10475.2003. J Neurosci. 2003. PMID: 14627631 Free PMC article. - Human gamma-aminobutyric acid type B receptors are differentially expressed and regulate inwardly rectifying K+ channels.
Kaupmann K, Schuler V, Mosbacher J, Bischoff S, Bittiger H, Heid J, Froestl W, Leonhard S, Pfaff T, Karschin A, Bettler B. Kaupmann K, et al. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14991-6. doi: 10.1073/pnas.95.25.14991. Proc Natl Acad Sci U S A. 1998. PMID: 9844003 Free PMC article. - Reversal of disease-related pathologies in the fragile X mouse model by selective activation of GABAB receptors with arbaclofen.
Henderson C, Wijetunge L, Kinoshita MN, Shumway M, Hammond RS, Postma FR, Brynczka C, Rush R, Thomas A, Paylor R, Warren ST, Vanderklish PW, Kind PC, Carpenter RL, Bear MF, Healy AM. Henderson C, et al. Sci Transl Med. 2012 Sep 19;4(152):152ra128. doi: 10.1126/scitranslmed.3004218. Sci Transl Med. 2012. PMID: 22993295 Free PMC article.