A method for high efficiency YAC lipofection into murine embryonic stem cells - PubMed (original) (raw)

A method for high efficiency YAC lipofection into murine embryonic stem cells

J T Lee et al. Nucleic Acids Res. 1996.

Abstract

We describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryonic stem (ES) cells by lipofection. With a decreased DNA:cell ratio, increased concentration of condensing agents and altered culture conditions, this protocol reduces the requirement for YAC DNA to a few micrograms, improves the recovery of neomycin-resistant ES colonies and increases the yield of clones containing both flanking vector markers and insert. These modifications enable generation of sufficient 'intact' transgenic clones for biological analysis with a single experiment.

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References

    1. Science. 1987 May 15;236(4803):806-12 - PubMed
    1. Science. 1993 Mar 26;259(5103):1904-7 - PubMed
    1. Nature. 1993 Mar 18;362(6417):205-6 - PubMed
    1. Nature. 1993 Mar 18;362(6417):258-61 - PubMed
    1. Curr Opin Genet Dev. 1995 Jun;5(3):342-8 - PubMed

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