Changes in the elastic properties of cholinergic synaptic vesicles as measured by atomic force microscopy - PubMed (original) (raw)

Changes in the elastic properties of cholinergic synaptic vesicles as measured by atomic force microscopy

D E Laney et al. Biophys J. 1997 Feb.

Abstract

Cholinergic synaptic vesicles from Torpedo californica have been probed with the atomic force microscope in aqueous buffers to map and measure their elastic properties. Elastic properties were mapped with a new atomic force microscope technique known as force mapping. Force mapping of vesicles showed that the centers of the vesicles are harder or stiffer than the peripheral areas in the three buffers that were investigated. These were an isoosmotic buffer, a hypoosmotic buffer, and an isoosmotic buffer with 5 mM CaCl2 added. The hardness of the vesicular centers was quantified by calculation of the elastic modulus. Elastic moduli were in the range of 2-13 x 10(5) Pa. Vesicular centers were hardest in calcium-containing buffer and softest in isoosmotic buffer. Hypotheses are presented for the composition and function of the hard centers.

PubMed Disclaimer

Similar articles

• Substructure and responses of cholinergic synaptic vesicles in the atomic force microscope.
  García RA, Laney DE, Parsons SM, Hansma HG. García RA, et al. J Neurosci Res. 1998 May 1;52(3):350-5. doi: 10.1002/(SICI)1097-4547(19980501)52:3<350::AID-JNR11>3.0.CO;2-A. J Neurosci Res. 1998. PMID: 9590443
• Transient increase of calcium in synaptic vesicles after stimulation.
  Parducz A, Dunant Y. Parducz A, et al. Neuroscience. 1993 Jan;52(1):27-33. doi: 10.1016/0306-4522(93)90178-i. Neuroscience. 1993. PMID: 8381925
• Proton NMR detection of acetylcholine status in synaptic vesicles.
  Stadler H, Füldner HH. Stadler H, et al. Nature. 1980 Jul 17;286(5770):293-4. doi: 10.1038/286293a0. Nature. 1980. PMID: 6250057
• Properties of biomolecules measured from atomic force microscope images: a review.
  Hansma HG, Kim KJ, Laney DE, Garcia RA, Argaman M, Allen MJ, Parsons SM. Hansma HG, et al. J Struct Biol. 1997 Jul;119(2):99-108. doi: 10.1006/jsbi.1997.3855. J Struct Biol. 1997. PMID: 9245749 Review.
• [Operator hypothesis in presynaptic membrane and vesicular hypothesis in synaptic vesicle (author's transl)].
  Ohsawa K. Ohsawa K. Tanpakushitsu Kakusan Koso. 1982 Feb;27(3):466-74. Tanpakushitsu Kakusan Koso. 1982. PMID: 6281853 Review. Japanese. No abstract available.

Cited by

• The roles of integrin β1 in phenotypic maintenance and dedifferentiation in chondroid cells differentiated from human adipose-derived stem cells.
  Luo S, Shi Q, Zha Z, Yao P, Lin H, Liu N, Wu H, Cai J, Sun S. Luo S, et al. Nanoscale Res Lett. 2013 Mar 24;8(1):136. doi: 10.1186/1556-276X-8-136. Nanoscale Res Lett. 2013. PMID: 23522347 Free PMC article.
• Trehalose-Induced Variation in Mechanical Properties of Vesicles in Aqueous Solution.
  Hur J, Park JW. Hur J, et al. J Membr Biol. 2015 Dec;248(6):1121-5. doi: 10.1007/s00232-015-9827-4. Epub 2015 Aug 2. J Membr Biol. 2015. PMID: 26233566
• Mechanical property estimation of sarcoma-relevant extracellular vesicles using transmission electron microscopy.
  Singh PK, Sarchet P, Hord C, Casadei L, Pollock R, Prakash S. Singh PK, et al. J Extracell Biol. 2024 Jul 4;3(7):e158. doi: 10.1002/jex2.158. eCollection 2024 Jul. J Extracell Biol. 2024. PMID: 38966868 Free PMC article.
• Cross-linked bioreducible layer-by-layer films for increased cell adhesion and transgene expression.
  Blacklock J, Sievers TK, Handa H, You YZ, Oupický D, Mao G, Möhwald H. Blacklock J, et al. J Phys Chem B. 2010 Apr 29;114(16):5283-91. doi: 10.1021/jp100486h. J Phys Chem B. 2010. PMID: 20369813 Free PMC article.
• Two kinesins transport cargo primarily via the action of one motor: implications for intracellular transport.
  Jamison DK, Driver JW, Rogers AR, Constantinou PE, Diehl MR. Jamison DK, et al. Biophys J. 2010 Nov 3;99(9):2967-77. doi: 10.1016/j.bpj.2010.08.025. Biophys J. 2010. PMID: 21044594 Free PMC article.

References

1. 1. Science. 1992 Sep 25;257(5078):1944-6 - PubMed
2. 1. Neuroimage. 1995 Mar;2(1):3-7 - PubMed
3. 1. Neuroscience. 1993 Jan;52(1):27-33 - PubMed
4. 1. J Neurochem. 1993 Jul;61(1):29-44 - PubMed
5. 1. Biophys J. 1993 Sep;65(3):992-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Europe PubMed Central
  • PubMed Central