Viral DNA sequences from incomplete particles of human adenovirus type 7 - PubMed (original) (raw)
Viral DNA sequences from incomplete particles of human adenovirus type 7
C Tibbetts. Cell. 1977 Sep.
Abstract
Large pools of empty viral capsids accumulate in cells infected by subgroup B human adenoviruses. Such infected cells also yield DNA-containing incomplete particles in larger quantities than cells infected with serotypes representing other adenovirus subgroups. DNA isolated from carefully purified classes of Ad7 incomplete particles was analyzed by restriction endonuclease cleavage, gel electrophoresis and electron microscopy. At least 90% of the DNA molecules in each sample consisted of sequences that extended from the left end of the viral genome map by variable lengths toward the right end. The average length of DNA is linearly related to the average buoyant density of the incomplete particles from which the DNA is isolated. The results indicate that each capsid contains one DNA molecule. There is also a specific association of the left end of the viral genome with assembled or assembling capsids. The characteristic distributions of Ad7 incomplete particles may result from intracellular pools of assembly intermediates in which the incompletely packaged DNA has been fragmented in vivo or by shear during preparative procedures.
Similar articles
- Synthesis of defective viral DNA in HeLa cells infected with adenovirus type 3.
Daniell E, Mullenbach T. Daniell E, et al. J Virol. 1978 Apr;26(1):61-70. doi: 10.1128/JVI.26.1.61-70.1978. J Virol. 1978. PMID: 650739 Free PMC article. - The nature of the DNA associated with incomplete particles of adenovirus type 2.
Khittoo G, Weber JM. Khittoo G, et al. J Gen Virol. 1981 Jun;54(Pt 2):343-55. doi: 10.1099/0022-1317-54-2-343. J Gen Virol. 1981. PMID: 6270253 - Isolation of incomplete adenovirus 16 particles containing viral and host cell DNA.
Hammarskjöld ML, Winberg G, Norrby E, Wadell G. Hammarskjöld ML, et al. Virology. 1977 Oct 15;82(2):449-61. doi: 10.1016/0042-6822(77)90018-6. Virology. 1977. PMID: 919347 No abstract available. - [Analysis of adenovirus genome by restriction endonuclease (author's transl)].
Sekikawa K, Fujinaga K. Sekikawa K, et al. Tanpakushitsu Kakusan Koso. 1977 Jul 1;22(8):1003-11. Tanpakushitsu Kakusan Koso. 1977. PMID: 337396 Review. Japanese. No abstract available. - Molecular biological characterization of adenovirus DNA.
Berencsi G, Nász I. Berencsi G, et al. Acta Microbiol Immunol Hung. 1998;45(3-4):297-304. Acta Microbiol Immunol Hung. 1998. PMID: 9873935 Review.
Cited by
- RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.
Ismail AM, Saha A, Lee JS, Painter DF, Chen Y, Singh G, Condezo GN, Chodosh J, San Martín C, Rajaiya J. Ismail AM, et al. PLoS Pathog. 2022 Jun 16;18(6):e1010588. doi: 10.1371/journal.ppat.1010588. eCollection 2022 Jun. PLoS Pathog. 2022. PMID: 35709296 Free PMC article. - Components of Adenovirus Genome Packaging.
Ahi YS, Mittal SK. Ahi YS, et al. Front Microbiol. 2016 Sep 23;7:1503. doi: 10.3389/fmicb.2016.01503. eCollection 2016. Front Microbiol. 2016. PMID: 27721809 Free PMC article. Review. - Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.
Condezo GN, Marabini R, Ayora S, Carazo JM, Alba R, Chillón M, San Martín C. Condezo GN, et al. J Virol. 2015 Sep;89(18):9653-64. doi: 10.1128/JVI.01453-15. Epub 2015 Jul 15. J Virol. 2015. PMID: 26178997 Free PMC article. - Identification and characterization of a DNA binding domain on the adenovirus IVa2 protein.
Christensen JB, Ewing SG, Imperiale MJ. Christensen JB, et al. Virology. 2012 Nov 10;433(1):124-30. doi: 10.1016/j.virol.2012.07.013. Epub 2012 Aug 9. Virology. 2012. PMID: 22884292 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources