Induction of Bip mRNA upon programmed cell death of differentiated PC12 cells as well as rat sympathetic neurons - PubMed (original) (raw)
Induction of Bip mRNA upon programmed cell death of differentiated PC12 cells as well as rat sympathetic neurons
T Aoki et al. J Biochem. 1997 Jan.
Free article
Abstract
We have found that expression of the Bip (immunoglobulin heavy chain binding protein)/GRP78 (glucose regulated protein 78) gene is markedly enhanced specifically among the heat shock protein (HSP) 70 gene family during the neuronal cell death of PC12 (22a) cells, that is induced by removal of nerve growth factor (NGF) and blocked by a transcription inhibitor, actinomycin D. The Bip mRNA induction is suppressed when the NGF-deprivation-dependent cell death of PC12 (22a) cells is inhibited by cAMP, cycloheximide or high K+. The Ca2+ ionophore, A23187, caused neuronal cell death accompanied by up-regulation of Bip, HSP90, and HSP70 mRNAs. In addition, a chelator of intracellular Ca2+ (BAPTA) elevated Bip mRNA and induced cell death in a low Ca2+ medium. Alterations of intracellular calcium homeostasis thus appear to induce Bip mRNA expression as well as apoptosis in PC12 (22a) cells. However, release of Ca2+ from intracellular stores by thapsigargin induced Bip mRNA expression but not cell death, indicating that Bip mRNA induction is not sufficient for neuronal death. Induction of Bip mRNA in association with apoptosis was also observed for NGF-deprived sympathetic ganglion cells in primary culture. These lines of evidence suggest that selective induction of Bip mRNA may play an important role in the programmed cell death of neurons deprived of neurotrophic factors and could be a landmark of the neuronal programmed cell death.
Similar articles
- Nerve growth factor withdrawal-induced cell death in neuronal PC12 cells resembles that in sympathetic neurons.
Mesner PW, Winters TR, Green SH. Mesner PW, et al. J Cell Biol. 1992 Dec;119(6):1669-80. doi: 10.1083/jcb.119.6.1669. J Cell Biol. 1992. PMID: 1469055 Free PMC article. - Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor.
Deckwerth TL, Johnson EM Jr. Deckwerth TL, et al. J Cell Biol. 1993 Dec;123(5):1207-22. doi: 10.1083/jcb.123.5.1207. J Cell Biol. 1993. PMID: 7503996 Free PMC article. - Depletion of intracellular Ca2+ store itself may be a major factor in thapsigargin-induced ER stress and apoptosis in PC12 cells.
Yoshida I, Monji A, Tashiro K, Nakamura K, Inoue R, Kanba S. Yoshida I, et al. Neurochem Int. 2006 Jun;48(8):696-702. doi: 10.1016/j.neuint.2005.12.012. Epub 2006 Feb 14. Neurochem Int. 2006. PMID: 16481070 - Oxygen toxicity induces apoptosis in neuronal cells.
Satoh T, Enokido Y, Kubo T, Yamada M, Hatanaka H. Satoh T, et al. Cell Mol Neurobiol. 1998 Dec;18(6):649-66. doi: 10.1023/a:1020633919115. Cell Mol Neurobiol. 1998. PMID: 9876872 Review. - Use of cultured neurons and neuronal cell lines to study morphological, biochemical, and molecular changes occurring in cell death.
Mills JC, Wang S, Erecińska M, Pittman RN. Mills JC, et al. Methods Cell Biol. 1995;46:217-42. doi: 10.1016/s0091-679x(08)61931-7. Methods Cell Biol. 1995. PMID: 7609653 Review. No abstract available.
Cited by
- Convergent evolution of heat-inducibility during subfunctionalization of the Hsp70 gene family.
Krenek S, Schlegel M, Berendonk TU. Krenek S, et al. BMC Evol Biol. 2013 Feb 21;13:49. doi: 10.1186/1471-2148-13-49. BMC Evol Biol. 2013. PMID: 23433225 Free PMC article. - Therapeutic potential of mood stabilizers lithium and valproic acid: beyond bipolar disorder.
Chiu CT, Wang Z, Hunsberger JG, Chuang DM. Chiu CT, et al. Pharmacol Rev. 2013 Jan 8;65(1):105-42. doi: 10.1124/pr.111.005512. Print 2013 Jan. Pharmacol Rev. 2013. PMID: 23300133 Free PMC article. Review. - Molecular actions and therapeutic potential of lithium in preclinical and clinical studies of CNS disorders.
Chiu CT, Chuang DM. Chiu CT, et al. Pharmacol Ther. 2010 Nov;128(2):281-304. doi: 10.1016/j.pharmthera.2010.07.006. Epub 2010 Aug 10. Pharmacol Ther. 2010. PMID: 20705090 Free PMC article. Review. - Calcium signaling and cytotoxicity.
Kass GE, Orrenius S. Kass GE, et al. Environ Health Perspect. 1999 Feb;107 Suppl 1(Suppl 1):25-35. doi: 10.1289/ehp.99107s125. Environ Health Perspect. 1999. PMID: 10229704 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous