Docetaxel enhances the expression of E-cadherin and carcinoembryonic antigen (CEA) on human colon cancer cell lines in vitro - PubMed (original) (raw)
. 1997 Jan-Feb;17(1A):7-12.
Affiliations
- PMID: 9066624
Docetaxel enhances the expression of E-cadherin and carcinoembryonic antigen (CEA) on human colon cancer cell lines in vitro
K Eckert et al. Anticancer Res. 1997 Jan-Feb.
Abstract
The in vitro effects of docetaxel on the human colon carcinoma cell lines-HT-29 and SW620 were studied by measuring the expression of the differentiation markers E-cadherin and CEA with a recently developed immunohistochemical assay. Docetaxel was shown to increase the expression of E-cadherin and CEA in the low concentration range of 3 x 10(-10) M - 3 x 10(-9) M for HT-29 cells and 0.6 - 3 x 10(-9) M for SW620 cells. Docetaxel strongly increased CEA (270%) and E-cadherin (160%) expression in HT-29 cells, compared with both marker expression in SW620 cells (60%). E-cadherin and CEA expression were found to be inversely correlated to the antiproliferating potential of docetaxel, which inhibits the cell proliferation of HT-29 cells at an IC50 of 6 x 10(-10) M and of SW620 cells at 8 x 10(-10) M. The data suggest, that in addition to its antiproliferative activity docetaxel also exhibits a strong differentiation inducing capacity at concentrations < 10(-9) M in vitro. These docetaxel effects were more pronounced on the differentiated HT-29 cells than on the poorly differentiated SW620 cells.