Complex evolution of a salmonid microsatellite locus and its consequences in inferring allelic divergence from size information - PubMed (original) (raw)
Comparative Study
Complex evolution of a salmonid microsatellite locus and its consequences in inferring allelic divergence from size information
B Angers et al. Mol Biol Evol. 1997 Mar.
Abstract
Sequence analysis of 78 alleles of a microsatellite locus was resolved at various taxonomic levels among salmonid fishes to test for congruence between size and sequence information. Allelic variability of this locus involved changes in number of dinucleotide repeats as expected for microsatellite loci. However, additional mutational events involving large indels and base substitutions also occurred frequently among and within species. These caused the evolution of different imperfect microsatellite variants, from an ancestral perfect one, which was undetectable from allelic size information only. Alleles of the same length resulting from different mutational events were also observed among and within species. Altogether, these results revealed the incongruence between size and number of mutation events, implying that it may be imprecise to interpret mutational rates and relationships on the basis of size information alone in interspecific comparisons and, to a lesser extent, within species.
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