Molecular dissection of the AGAMOUS control region shows that cis elements for spatial regulation are located intragenically - PubMed (original) (raw)

Molecular dissection of the AGAMOUS control region shows that cis elements for spatial regulation are located intragenically

L E Sieburth et al. Plant Cell. 1997 Mar.

Abstract

AGAMOUS (AG) is an Arabidopsis MADS box gene required for the normal development of the internal two whorls of the flower. AG RNA accumulates in distinct patterns early and late in flower development, and several genes have been identified as regulators of AG gene expression based on altered AG RNA accumulation in mutants. To understand AG regulatory circuits, we are now identifying cis regulatory domains by characterizing AG::beta-glucuronidase (GUS) gene fusions. These studies show that a normal AG::GUS staining pattern is conferred by a 9.8-kb region encompassing 6 kb of upstream sequences and 3.8 kb of intragenic sequences. Constructs lacking the 3.8-kb intragenic sequences confer a GUS staining pattern that deviates both spatially and temporally from normal AG expression. The GUS staining patterns in the mutants for the three negative regulators of AG, apetala2, leunig, and curly leaf, showed the predicted change of expression for the construct containing the intragenic sequences, but no significant change was observed for the constructs lacking this intragenic region. These results suggest that intragenic sequences are essential for AG regulation and that these intragenic sequences contain the ultimate target sites for at least some of the known regulatory molecules.

PubMed Disclaimer

Cited by

Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.
Kim HB, Cho JI, Ryoo N, Qu S, Wang GL, Jeon JS. Kim HB, et al. Mol Cells. 2012 Jan;33(1):61-9. doi: 10.1007/s10059-012-2212-6. Epub 2011 Nov 29. Mol Cells. 2012. PMID: 22134722 Free PMC article.
Cytokinin acts through the auxin influx carrier AUX1 to regulate cell elongation in the root.
Street IH, Mathews DE, Yamburkenko MV, Sorooshzadeh A, John RT, Swarup R, Bennett MJ, Kieber JJ, Schaller GE. Street IH, et al. Development. 2016 Nov 1;143(21):3982-3993. doi: 10.1242/dev.132035. Epub 2016 Oct 3. Development. 2016. PMID: 27697901 Free PMC article.
A cryptic natural variant allele of BYPASS2 suppresses the bypass1 mutant phenotype.
Cummins AJ, Siler CJ, Olson JM, Kaur A, Hamdani AK, Olson LK, Dilkes BP, Sieburth LE. Cummins AJ, et al. Plant Physiol. 2023 May 31;192(2):1016-1027. doi: 10.1093/plphys/kiad124. Plant Physiol. 2023. PMID: 36905371 Free PMC article.
Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions.
Ascenzi R, Ulker B, Todd JJ, Sowinski DA, Schimeneck CR, Allen GC, Weissinger AK, Thompson WF. Ascenzi R, et al. Transgenic Res. 2003 Jun;12(3):305-18. doi: 10.1023/a:1023310118231. Transgenic Res. 2003. PMID: 12779119
Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta.
Cazzonelli CI, McCallum EJ, Lee R, Botella JR. Cazzonelli CI, et al. Transgenic Res. 2005 Dec;14(6):941-67. doi: 10.1007/s11248-005-2539-2. Transgenic Res. 2005. PMID: 16315097

References

1. Cell. 1992 Oct 2;71(1):133-43 - PubMed
1. Plant Cell. 1994 Mar;6(3):333-349 - PubMed
1. Cell. 1991 Jun 14;65(6):991-1002 - PubMed
1. Curr Opin Cell Biol. 1995 Jun;7(3):399-405 - PubMed
1. Plant Cell. 1991 Aug;3(8):749-58 - PubMed

Molecular dissection of the AGAMOUS control region shows that cis elements for spatial regulation are located intragenically - PubMed (original) (raw)