Thymus-independent generation of NK1+ T cells in vitro from fetal liver precursors - PubMed (original) (raw)

. 1997 Apr 15;158(8):3682-9.

Affiliations

PMID: 9103431

Thymus-independent generation of NK1+ T cells in vitro from fetal liver precursors

M Shimamura et al. J Immunol. 1997.

Abstract

NK1.1+TCR alpha beta+ (NK1+) T cells are an unusual subset of mouse TCR alpha beta+ cells found primarily in adult thymus and liver. In contrast to conventional TCR alpha beta+ cells, NK1+ T cells have a TCR repertoire that is highly skewed to V alpha14 and to Vbeta8, -7, and -2. The developmental origin and ligand specificity of NK1+ T cells are controversial. We show here that NK1+ T cells with a typically biased V alpha and V beta repertoire develop in cytokine-supplemented suspension cultures of fetal liver established from either normal or athymic mice. Furthermore, NK1+ T cell development in fetal liver cultures is abrogated in beta2m-deficient mice (which lack MHC class I and other related molecules) and can be partially inhibited by the presence of anti-CD1 mAbs. Moreover, mixing experiments indicate that recombination-deficient SCID fetal liver cells can reconstitute NK1+ T cell development in beta2m-deficient fetal liver cultures. Collectively, our data demonstrate that NK1+ T cells can develop extrathymically from fetal liver precursors and that a beta2m-associated ligand (putatively CD1) present on nonlymphoid cells is essential for their positive selection and/or expansion.

PubMed Disclaimer

Cited by

A natural killer T (NKT) cell developmental pathway iInvolving a thymus-dependent NK1.1(-)CD4(+) CD1d-dependent precursor stage.
Pellicci DG, Hammond KJ, Uldrich AP, Baxter AG, Smyth MJ, Godfrey DI. Pellicci DG, et al. J Exp Med. 2002 Apr 1;195(7):835-44. doi: 10.1084/jem.20011544. J Exp Med. 2002. PMID: 11927628 Free PMC article.
Activation of invariant natural killer T cells stimulated with microbial α-mannosyl glycolipids.
Shimamura M, Yamamura M, Nabeshima T, Kitano N, van den Elzen P, Yesilkaya H, Andrew P, Illarionov P. Shimamura M, et al. Sci Rep. 2017 Aug 29;7(1):9703. doi: 10.1038/s41598-017-10309-x. Sci Rep. 2017. PMID: 28852174 Free PMC article.
Innate self recognition by an invariant, rearranged T-cell receptor and its immune consequences.
Stanic AK, Park JJ, Joyce S. Stanic AK, et al. Immunology. 2003 Jun;109(2):171-84. doi: 10.1046/j.1365-2567.2003.01657.x. Immunology. 2003. PMID: 12757612 Free PMC article. Review.
NIK-dependent RelB activation defines a unique signaling pathway for the development of V alpha 14i NKT cells.
Elewaut D, Shaikh RB, Hammond KJ, De Winter H, Leishman AJ, Sidobre S, Turovskaya O, Prigozy TI, Ma L, Banks TA, Lo D, Ware CF, Cheroutre H, Kronenberg M. Elewaut D, et al. J Exp Med. 2003 Jun 16;197(12):1623-33. doi: 10.1084/jem.20030141. J Exp Med. 2003. PMID: 12810685 Free PMC article.

Thymus-independent generation of NK1+ T cells in vitro from fetal liver precursors - PubMed (original) (raw)