IL-5 secretion by allergen-stimulated CD4+ T cells in primary culture: relationship to expression of allergic disease - PubMed (original) (raw)
Comparative Study
IL-5 secretion by allergen-stimulated CD4+ T cells in primary culture: relationship to expression of allergic disease
S Till et al. J Allergy Clin Immunol. 1997 Apr.
Abstract
Background: IL-5-producing allergen-specific T cells are thought to play a prominent role in the pathogenesis of allergic inflammation. We hypothesized that T cell allergen-driven IL-5 synthesis is elevated in patients with atopic disease as compared with that in atopic patients free of disease and nonatopic control subjects.
Objectives: The purpose of this study was to compare IL-5 and interferon-gamma (IFN-gamma) secretion and proliferation by peripheral blood T cells from sensitized atopic patients with asthma, rhinitis, and no symptoms and from nonatopic control subjects in response to the allergen Dermatophagoides pteronyssinus (Der p) and the control recall antigen Mycobacterium tuberculosis purified protein derivative (PPD).
Methods: To measure allergen-induced IL-5 production and proliferation, we developed a short-term culture technique that required a single antigenic stimulation of freshly isolated peripheral blood mononuclear cells (PBMC). With this technique, we measured Der p- and PPD-induced IL-5 production and proliferation in PBMC from atopic patients with asthma who were allergic to Der p, atopic patients with rhinitis, atopic patients with no symptoms, and a group of nonatopic normal control subjects. In four experiments, CD4+ or CD8+ T cells were depleted from PBMC to confirm that IL-5 synthesis was T cell dependent.
Results: T cell IL-5 production, but not IFN-gamma production, in response to Der p was elevated in atopic patients with asthma and atopic patients with rhinitis compared with findings in atopic patients with no symptoms or nonatopic control subjects. IL-5 production was abrogated by depletion of CD4+, but not CD8+, T cells. In subjects with asthma, allergen-driven IL-5 production correlated with bronchial hyperreactivity. Allergen-induced proliferation was also higher in patients with asthma than in atopic subjects with no symptoms or nonatopic controls. T cell IL-5 and IFN-gamma production and proliferation in response to PPD were similar regardless of atopic status or disease.
Conclusions: Elevated IL-5 production is a characteristic of allergen-specific peripheral blood CD4+ T cells from sensitized patients with atopic disease but not atopy per se.
Similar articles
- IL-13 production by allergen-stimulated T cells is increased in allergic disease and associated with IL-5 but not IFN-gamma expression.
Till S, Durham S, Dickason R, Huston D, Bungre J, Walker S, Robinson D, Kay AB, Corrigan C. Till S, et al. Immunology. 1997 May;91(1):53-7. doi: 10.1046/j.1365-2567.1997.00218.x. Immunology. 1997. PMID: 9203965 Free PMC article. - IL-5 production by bronchoalveolar lavage and peripheral blood mononuclear cells in asthma and atopy.
Tang C, Rolland JM, Ward C, Quan B, Walters EH. Tang C, et al. Eur Respir J. 1997 Mar;10(3):624-32. Eur Respir J. 1997. PMID: 9072996 - Differential regulation of allergen-specific T(H2)- but not T(H1)-type responses by alveolar macrophages in atopic asthma.
Tang C, Rolland JM, Ward C, Thien F, Li X, Gollant S, Walters EH. Tang C, et al. J Allergy Clin Immunol. 1998 Sep;102(3):368-75. doi: 10.1016/s0091-6749(98)70122-8. J Allergy Clin Immunol. 1998. PMID: 9768575 - Role of allergen-specific T-follicular helper cells in immunotherapy.
Yao Y, Wang ZC, Yu D, Liu Z. Yao Y, et al. Curr Opin Allergy Clin Immunol. 2018 Dec;18(6):495-501. doi: 10.1097/ACI.0000000000000480. Curr Opin Allergy Clin Immunol. 2018. PMID: 30124489 Review. - Immune tolerance and protection against allergic sensitization.
Holt PG. Holt PG. Allergy. 1995;50(25 Suppl):34-6. doi: 10.1111/j.1398-9995.1995.tb04274.x. Allergy. 1995. PMID: 7677232 Review.
Cited by
- Construction by artificial intelligence and immunovalidation of hypoallergenic mite allergen Der f 36 vaccine.
Qin QZ, Tang J, Wang CY, Xu ZQ, Tian M. Qin QZ, et al. Front Immunol. 2024 Mar 27;15:1325998. doi: 10.3389/fimmu.2024.1325998. eCollection 2024. Front Immunol. 2024. PMID: 38601166 Free PMC article. - Decrease of IL-5 Production by Naive T Cells Cocultured with IL-18-Producing BCG-Pulsed Dendritic Cells from Patients Allergic to House Dust Mite.
Kowalewicz-Kulbat M, Szpakowski P, Krawczyk KT, Kowalski ML, Kosinski S, Biet F, Rudnicka W, Locht C. Kowalewicz-Kulbat M, et al. Vaccines (Basel). 2021 Mar 18;9(3):277. doi: 10.3390/vaccines9030277. Vaccines (Basel). 2021. PMID: 33803752 Free PMC article. - Aberrant Th2 Immune Responses Are Associated With a Reduced Frequency of IL-35-Induced Regulatory T Cells After Allergen Exposure in Patients With Allergic Asthma.
Wang W, Wei C, Cheng Z, Yang J. Wang W, et al. Allergy Asthma Immunol Res. 2020 Nov;12(6):1029-1045. doi: 10.4168/aair.2020.12.6.1029. Allergy Asthma Immunol Res. 2020. PMID: 32935493 Free PMC article. - The levels of CD4+CD25+ regulatory T cells in patients with allergic rhinitis.
Shaoqing Y, Yinjian C, Zhiqiang Y, Ruxin Z, Na C, Rongming G. Shaoqing Y, et al. Allergol Select. 2018 Sep 1;2(1):144-150. doi: 10.5414/ALX01782E. eCollection 2018. Allergol Select. 2018. PMID: 31826046 Free PMC article. - Effective antigen presentation to helper T cells by human eosinophils.
Farhan RK, Vickers MA, Ghaemmaghami AM, Hall AM, Barker RN, Walsh GM. Farhan RK, et al. Immunology. 2016 Dec;149(4):413-422. doi: 10.1111/imm.12658. Epub 2016 Sep 20. Immunology. 2016. PMID: 27502559 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials