Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley - PubMed (original) (raw)

Run-on transcription of defense-related genes in nuclei isolated from cultured parsley cells 3 or 6 hr after treatment with water (“untreated”), crude cell-wall elicitor (50 μg/ml), amphotericin B (50 μM), nystatin (50 μM), or digitonin (50 μM). The homologous cDNA probes used for hybridization encode phenylalanine ammonia-lyase (pal), 4-coumarate:CoA ligase (4cl), chalcone synthase (chs), _S_-adenosyl-

-methionine:bergaptol _O_-methyltransferase (bmt), tyrosine decarboxylase (tyrdc), hydroxyproline-rich glycoprotein (hrgp), anionic peroxidase (per), _S_-adenosyl-

-homocystein hydrolase (shh), _S_-adenosyl-

-methionine synthetase 1 (sms1), intracellular pathogenesis-related proteins 1 and 2 (pr1 and pr2), proteins of unknown function (eli3, eli6, eli7, eli8, eli10, eli12, eli16, eli17, rep), polyubiquitin (ubi4), and ribosomal 18S RNA (r18s).