CREB is activated by UVC through a p38/HOG-1-dependent protein kinase - PubMed (original) (raw)

CREB is activated by UVC through a p38/HOG-1-dependent protein kinase

M Iordanov et al. EMBO J. 1997.

Abstract

Changes in environmental conditions such as the addition of growth factors or irradiation of cells in culture first affect immediate response genes. We have shown previously that short wavelength UV irradiation (UVC) elicits massive activation of several growth factor receptor-dependent pathways. At the level of the immediate response gene c-fos, these pathways activate the transcription factor complex serum response factor (SRF)-p62TCF which mediates part of the UV-induced transcriptional response. These studies have, however, suggested that more that one pathway is required for full UV responsiveness of c-fos. Using appropriate promoter mutations and dominant-negative cAMP response element (CRE)-binding protein (CREB), we now find that UVC-induced transcriptional activation depends also on the CRE at position -60 of the c-fos promoter and on the functionality of a CREB. Upon UV irradiation, CREB and ATF-1 are phosphorylated at serines 133 and 63, respectively, preceded by and dependent on activation of p38/RK/HOG-1 and of a p38/RK/HOG-1-dependent p108 CREB kinase. Although p90RSK1 and MAPKAP kinase 2 are also activated by UV, p90RSK1 does not, at least not decisively, participate in this signalling pathway to CREB and ATF-1 as it is not p38/RK/HOG-1 dependent, and CREB is a poor substrate for MAPKAP kinase 2 in vitro. On the basis of resistance to the growth factor receptor inhibitor suramin and of several types of cross-refractoriness experiments, the UVC-induced CREB/ATF-1 phosphorylation represents an as yet unrecognized route of UVC-induced signal transduction, independent of suramin-inhibitable growth factor receptors and different from the Erk 1,2-p62TCF pathway.

PubMed Disclaimer

Cited by

Regulation of PRAK subcellular location by p38 MAP kinases.
New L, Jiang Y, Han J. New L, et al. Mol Biol Cell. 2003 Jun;14(6):2603-16. doi: 10.1091/mbc.e02-08-0538. Epub 2003 Mar 20. Mol Biol Cell. 2003. PMID: 12808055 Free PMC article.
p38 mitogen-activated kinase is a bidirectional regulator of human fibroblast collagenase-1 induction by three-dimensional collagen lattices.
Xu J, Clark RA, Parks WC. Xu J, et al. Biochem J. 2001 Apr 15;355(Pt 2):437-47. doi: 10.1042/0264-6021:3550437. Biochem J. 2001. PMID: 11284732 Free PMC article.
Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement.
Teng LL, Lu GL, Chiou LC, Lin WS, Cheng YY, Hsueh TE, Huang YC, Hwang NH, Yeh JW, Liao RM, Fan SZ, Yen JH, Fu TF, Tsai TF, Wu MS, Wang PY. Teng LL, et al. PLoS Biol. 2019 Mar 18;17(3):e2007097. doi: 10.1371/journal.pbio.2007097. eCollection 2019 Mar. PLoS Biol. 2019. PMID: 30883547 Free PMC article.
Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB.
Deak M, Clifton AD, Lucocq LM, Alessi DR. Deak M, et al. EMBO J. 1998 Aug 3;17(15):4426-41. doi: 10.1093/emboj/17.15.4426. EMBO J. 1998. PMID: 9687510 Free PMC article.

References

1. Cell. 1994 Mar 25;76(6):1025-37 - PubMed
1. Cell. 1993 Nov 19;75(4):765-78 - PubMed
1. Curr Opin Genet Dev. 1994 Feb;4(1):96-101 - PubMed
1. Cell. 1994 Jun 3;77(5):713-25 - PubMed
1. Neuron. 1994 Jun;12(6):1207-21 - PubMed

CREB is activated by UVC through a p38/HOG-1-dependent protein kinase - PubMed (original) (raw)