Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage - PubMed (original) (raw)

. 1997 Apr 1;99(7):1534-45.

doi: 10.1172/JCI119316.

L Dahlberg, M Ionescu, A Reiner, R Bourne, C Rorabeck, P Mitchell, J Hambor, O Diekmann, H Tschesche, J Chen, H Van Wart, A R Poole

Affiliations

• PMID: 9119997
• PMCID: PMC507973
• DOI: 10.1172/JCI119316

Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage

R C Billinghurst et al. J Clin Invest. 1997.

Abstract

We demonstrate the direct involvement of increased collagenase activity in the cleavage of type II collagen in osteoarthritic human femoral condylar cartilage by developing and using antibodies reactive to carboxy-terminal (COL2-3/4C(short)) and amino-terminal (COL2-1/4N1) neoepitopes generated by cleavage of native human type II collagen by collagenase matrix metalloproteinase (MMP)-1 (collagenase-1), MMP-8 (collagenase-2), and MMP-13 (collagenase-3). A secondary cleavage followed the initial cleavage produced by these recombinant collagenases. This generated neoepitope COL2-1/4N2. There was significantly more COL2-3/4C(short) neoepitope in osteoarthritis (OA) compared to adult nonarthritic cartilages as determined by immunoassay of cartilage extracts. A synthetic preferential inhibitor of MMP-13 significantly reduced the unstimulated release in culture of neoepitope COL2-3/4C(short) from human osteoarthritic cartilage explants. These data suggest that collagenase(s) produced by chondrocytes is (are) involved in the cleavage and denaturation of type II collagen in articular cartilage, that this is increased in OA, and that MMP-13 may play a significant role in this process.

PubMed Disclaimer

Similar articles

• Selective enhancement of collagenase-mediated cleavage of resident type II collagen in cultured osteoarthritic cartilage and arrest with a synthetic inhibitor that spares collagenase 1 (matrix metalloproteinase 1).
  Dahlberg L, Billinghurst RC, Manner P, Nelson F, Webb G, Ionescu M, Reiner A, Tanzer M, Zukor D, Chen J, van Wart HE, Poole AR. Dahlberg L, et al. Arthritis Rheum. 2000 Mar;43(3):673-82. doi: 10.1002/1529-0131(200003)43:3<673::AID-ANR25>3.0.CO;2-8. Arthritis Rheum. 2000. PMID: 10728762
• Comparison of the degradation of type II collagen and proteoglycan in nasal and articular cartilages induced by interleukin-1 and the selective inhibition of type II collagen cleavage by collagenase.
  Billinghurst RC, Wu W, Ionescu M, Reiner A, Dahlberg L, Chen J, van Wart H, Poole AR. Billinghurst RC, et al. Arthritis Rheum. 2000 Mar;43(3):664-72. doi: 10.1002/1529-0131(200003)43:3<664::AID-ANR24>3.0.CO;2-D. Arthritis Rheum. 2000. PMID: 10728761
• The role of type IX collagen in osteoarthritis and rheumatoid arthritis.
  Diab M. Diab M. Orthop Rev. 1993 Feb;22(2):165-70. Orthop Rev. 1993. PMID: 8451069 Review.
• Is collagenase (matrix metalloproteinase-1) necessary for bone and other connective tissue remodeling?
  Krane SM. Krane SM. Clin Orthop Relat Res. 1995 Apr;(313):47-53. Clin Orthop Relat Res. 1995. PMID: 7641497 Review.

Cited by

• Articular Cartilage of the Human Knee Joint: In Vivo Multicomponent T2 Analysis at 3.0 T.
  Liu F, Choi KW, Samsonov A, Spencer RG, Wilson JJ, Block WF, Kijowski R. Liu F, et al. Radiology. 2015 Nov;277(2):477-88. doi: 10.1148/radiol.2015142201. Epub 2015 May 25. Radiology. 2015. PMID: 26024307 Free PMC article.
• Structural insights into triple-helical collagen cleavage by matrix metalloproteinase 1.
  Manka SW, Carafoli F, Visse R, Bihan D, Raynal N, Farndale RW, Murphy G, Enghild JJ, Hohenester E, Nagase H. Manka SW, et al. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12461-6. doi: 10.1073/pnas.1204991109. Epub 2012 Jul 3. Proc Natl Acad Sci U S A. 2012. PMID: 22761315 Free PMC article.
• Secretory leukocyte protease inhibitor suppresses the inflammation and joint damage of bacterial cell wall-induced arthritis.
  Song Xy, Zeng L, Jin W, Thompson J, Mizel DE, Lei K, Billinghurst RC, Poole AR, Wahl SM. Song Xy, et al. J Exp Med. 1999 Aug 16;190(4):535-42. doi: 10.1084/jem.190.4.535. J Exp Med. 1999. PMID: 10449524 Free PMC article.
• The shed ectodomain of collagen XVII/BP180 is targeted by autoantibodies in different blistering skin diseases.
  Schumann H, Baetge J, Tasanen K, Wojnarowska F, Schäcke H, Zillikens D, Bruckner-Tuderman L. Schumann H, et al. Am J Pathol. 2000 Feb;156(2):685-95. doi: 10.1016/S0002-9440(10)64772-4. Am J Pathol. 2000. PMID: 10666397 Free PMC article.
• Cartilage biology, pathology, and repair.
  Umlauf D, Frank S, Pap T, Bertrand J. Umlauf D, et al. Cell Mol Life Sci. 2010 Dec;67(24):4197-211. doi: 10.1007/s00018-010-0498-0. Epub 2010 Aug 25. Cell Mol Life Sci. 2010. PMID: 20734104 Free PMC article. Review.

References

1. 1. Crit Rev Oral Biol Med. 1993;4(2):197-250 - PubMed
2. 1. Matrix. 1992 Dec;12(6):475-80 - PubMed
3. 1. Biochemistry. 1966 Oct;5(10):3123-30 - PubMed
4. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
5. 1. J Bone Joint Surg Am. 1971 Apr;53(3):523-37 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Society for Clinical Investigation
  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information