Generation of NK1.1+ T cell antigen receptor alpha/beta+ thymocytes associated with intact thymic structure - PubMed (original) (raw)

Comparative Study

Generation of NK1.1+ T cell antigen receptor alpha/beta+ thymocytes associated with intact thymic structure

K Nakagawa et al. Proc Natl Acad Sci U S A. 1997.

Erratum in

Proc Natl Acad Sci U S A 1997 May 27;94(11):5979

Abstract

The development of T cells within the thymus is largely dependent on intact cortical and medullary epithelial cells. However, it has been reported that positive selection of natural killer antigen 1.1+ (NK1.1+) T cell antigen receptor (TCR)-alpha/beta+ thymocytes recently identified among CD4+8- and CD4-8- subpopulations is attributable to major histocompatibility complex class Ib ligands expressed on bone marrow (BM)-derived components in the thymus. In the present study, we investigated generation of NK1.1+ TCR-alpha/beta+ cells in the thymus of the aly/aly mouse which lacks lymph nodes and Peyer's patches and shows abnormalities of thymic and splenic structure. We found that the proportion of the NK1.1+ TCR-alpha/beta+ thymocytes was extremely low in these mice as compared with aly/+ and normal C57BL/6 mice. Thymic reconstitution by BM cells from aly/+ mice that possess a normal population of NK1.1+ TCR-alpha/beta+ cell population did not restore the NK1.1+ TCR-alpha/beta+ cell population in the thymus of lethally irradiated aly/aly mouse. When deoxyguanosine-treated fetal thymi from (B6 x B10.G)F1 mice were transplanted to aly/aly mice that had been thymectomized and reconstituted with BM cells of aly/aly mice, normal proportions of the NK1.1+ TCR-alpha/beta+ thymocytes were present in the thymus grafts. These findings demonstrate that the development of NK1.1+ TCR-alpha/beta+ thymocytes is accomplished under the influence not only of BM-derived components, but also of irradiation-resistant or deoxyguanosine-resistant components and an intact microenvironment of the thymus.

PubMed Disclaimer

Figures

Figure 1

Proportion of NK1.1+ TCR-α/β+ cells in lymphoid tissues from aly/aly, aly/+ and B6 mice. Representative FACS profiles of NK1.1+ TCR-α/β+ (average ± SD) cells in the thymus (A) and spleen and BM (B) from three mice are shown. The actual numbers of NK1.1+ TCR-α/β+ thymocytes are shown below A.

Figure 2

Proportion of NK1.1+ TCR-α/β+ thymocytes in BM chimeras. Four kinds of BM chimeras prepared by reciprocal BM transplantation between aly/aly and aly/+ mice were analyzed for the population size of NK1.1+ TCR-α/β+ thymocytes (A). (B) Data for the average ± SD (number) of three to five chimeric mice.

Figure 3

Proportion of NK1.1+ TCR-α/β+ thymocytes in [aly/aly → B6.PL-Thy 1.1] BM chimeras. A representative profile of NK1.1+ TCR-α/β+ cells in whole thymocytes is shown from two separate experiments (Left). The origin of thymocytes was determined by anti-Thy 1 mAb (Right).

Figure 4

Histopathology of the thymus from aly/aly and aly/+ and chimeric mice. Thymic sections obtained from aly/+ (A, C,and E), aly/aly (B, D, and F), [aly/aly → aly/+] (G), and [aly/+ → aly/_aly_] (H) mice were stained either with hematoxylin/eosin (HE) (A, B, G, and H), ER-TR4 (C and D) or ER-TR5 (E and F). [HE staining (A, B, G, and H) = ×40; immunohistochemical staining (C, D, E, and F) = ×400.]

Figure 5

Proportion of NK1.1+ TCR-α/β+ cells in grafted thymi. Adult thymectomized aly/aly mice were reconstituted with aly/aly BM cells followed by grafting of (B6 × B10.G)F1 fetal thymi, and the proportion of the NK1.1+ TCR-α/β+ cells in the thymi was analyzed (A). Average ± SD (%) of three chimeras was indicated at the bottom. The origin of thymocytes was determined with anti-Dq mAb (B).

Cited by

NF-κB-inducing kinase contributes to normal development of cortical thymic epithelial cells: its possible role in shaping a proper T-cell repertoire.
Eshima K, Misawa K, Ohashi C, Noma H, Iwabuchi K. Eshima K, et al. Immunology. 2020 Jun;160(2):198-208. doi: 10.1111/imm.13186. Epub 2020 Apr 13. Immunology. 2020. PMID: 32145062 Free PMC article.
Differential requirement for Rel/nuclear factor kappa B family members in natural killer T cell development.
Sivakumar V, Hammond KJ, Howells N, Pfeffer K, Weih F. Sivakumar V, et al. J Exp Med. 2003 Jun 16;197(12):1613-21. doi: 10.1084/jem.20022234. J Exp Med. 2003. PMID: 12810684 Free PMC article.
A natural killer T (NKT) cell developmental pathway iInvolving a thymus-dependent NK1.1(-)CD4(+) CD1d-dependent precursor stage.
Pellicci DG, Hammond KJ, Uldrich AP, Baxter AG, Smyth MJ, Godfrey DI. Pellicci DG, et al. J Exp Med. 2002 Apr 1;195(7):835-44. doi: 10.1084/jem.20011544. J Exp Med. 2002. PMID: 11927628 Free PMC article.
Regulation of natural killer T-cell development by deubiquitinase CYLD.
Lee AJ, Zhou X, Chang M, Hunzeker J, Bonneau RH, Zhou D, Sun SC. Lee AJ, et al. EMBO J. 2010 May 5;29(9):1600-12. doi: 10.1038/emboj.2010.31. Epub 2010 Mar 11. EMBO J. 2010. PMID: 20224552 Free PMC article.
Differential dependence on nuclear factor-κB-inducing kinase among natural killer T-cell subsets in their development.
Noma H, Eshima K, Satoh M, Iwabuchi K. Noma H, et al. Immunology. 2015 Sep;146(1):89-99. doi: 10.1111/imm.12484. Epub 2015 Jun 25. Immunology. 2015. PMID: 25988531 Free PMC article.

References

1. Bendelac A. Curr Opin Immunol. 1995;7:367–374. - PubMed
1. MacDonald H R. J Exp Med. 1995;182:633–638. - PMC - PubMed
1. Arase H, Arase N, Ogasawara K, Good R A, Onoé K. Proc Natl Acad Sci USA. 1992;89:6506–6510. - PMC - PubMed
1. Arase H, Arase N, Nakagawa K, Good R A, Onoé K. Eur J Immunol. 1993;23:307–310. - PubMed
1. Yoshimoto T, Bendelac A, Watson C, Hu-Li J, Paul W E. Science. 1995;270:1845–1847. - PubMed

Generation of NK1.1+ T cell antigen receptor alpha/beta+ thymocytes associated with intact thymic structure - PubMed (original) (raw)