Na+/K+ ATPase in lower airway epithelium from cystic fibrosis and non-cystic-fibrosis lung - PubMed (original) (raw)
Comparative Study
. 1997 Mar 17;232(2):464-8.
doi: 10.1006/bbrc.1997.6200.
Affiliations
- PMID: 9125202
- DOI: 10.1006/bbrc.1997.6200
Comparative Study
Na+/K+ ATPase in lower airway epithelium from cystic fibrosis and non-cystic-fibrosis lung
D Peckham et al. Biochem Biophys Res Commun. 1997.
Abstract
The basolateral Na+/K+ ATPase plays a critical role in sodium reabsorption across airway epithelium. Nasal epithelium shows increased Na+/K+ ATPase activity in cystic fibrosis (CF) but Na+/K+ ATPase has not been characterized in human lung epithelium or compared in CF and normal lung. We measured 3[H] ouabain binding and Na+/K+ ATPase activity in human tracheal epithelium and compared Na/K ATPase activity in bronchial epithelium in CF and control subjects. In tracheal epithelium Na+/K+ ATPase pumps were abundant and of high 3[H] ouabain affinity (Kd 4.7 nM, Bmax 38 pmol/mg) and Na+/K+ ATPase activity was 55 +/- 8 nmol/mg protein/min. Bronchial epithelial Na+/K+ ATPase activity was twofold higher in CF patients than in controls. The increased Na+/K+ ATPase activity may contribute to the increased sodium reabsorption seen in cystic fibrosis.
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