Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein - PubMed (original) (raw)
. 1997 May 9;272(19):12249-52.
doi: 10.1074/jbc.272.19.12249.
Affiliations
- PMID: 9139664
- DOI: 10.1074/jbc.272.19.12249
Free article
Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein
R E Dodson et al. J Biol Chem. 1997.
Free article
Abstract
RNA-binding proteins containing KH domains are widely distributed. One KH domain protein of unknown function, vigilin (also known as the high density lipoprotein-binding protein), contains 14 KH domains and is ubiquitous in vertebrate cells. We previously used RNA gel mobility shift assays to describe an estrogen-inducible protein which binds specifically to a segment of the 3'-untranslated region (3'-UTR) of vitellogenin mRNA, an area which has been implicated in the estrogen-mediated stabilization of vitellogenin mRNA. Here we show that the vitellogenin mRNA-binding protein (VitRNABP) is vigilin. The VitRNABP was isolated as a 150-155-kDa protein on a vitellogenin mRNA 3'-UTR affinity column. Peptide microsequencing revealed that the purified protein was vigilin, a conclusion confirmed in Western blot analysis with antibodies to vigilin. Direct confirmation that vigilin is the VitRNABP was obtained from RNA gel mobility shift assays which demonstrated that antibodies to chicken vigilin supershifted the Xenopus VitRNABP band. Xenopus liver vigilin mRNA and the VitRNABP exhibited similar induction by estrogen, providing additional confirmation that vigilin is the estrogen-inducible protein which binds to the 3'-UTR of estrogen-stabilized vitellogenin mRNA. These data support a role for vigilin in the hormonal control of mRNA metabolism.
Similar articles
- Vigilin binding selectively inhibits cleavage of the vitellogenin mRNA 3'-untranslated region by the mRNA endonuclease polysomal ribonuclease 1.
Cunningham KS, Dodson RE, Nagel MA, Shapiro DJ, Schoenberg DR. Cunningham KS, et al. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12498-502. doi: 10.1073/pnas.220425497. Proc Natl Acad Sci U S A. 2000. PMID: 11050168 Free PMC article. - In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein.
Kanamori H, Dodson RE, Shapiro DJ. Kanamori H, et al. Mol Cell Biol. 1998 Jul;18(7):3991-4003. doi: 10.1128/MCB.18.7.3991. Mol Cell Biol. 1998. PMID: 9632784 Free PMC article. - An estrogen-inducible protein binds specifically to a sequence in the 3' untranslated region of estrogen-stabilized vitellogenin mRNA.
Dodson RE, Shapiro DJ. Dodson RE, et al. Mol Cell Biol. 1994 May;14(5):3130-8. doi: 10.1128/mcb.14.5.3130-3138.1994. Mol Cell Biol. 1994. PMID: 8164668 Free PMC article. - Regulation of pathways of mRNA destabilization and stabilization.
Dodson RE, Shapiro DJ. Dodson RE, et al. Prog Nucleic Acid Res Mol Biol. 2002;72:129-64. doi: 10.1016/s0079-6603(02)72069-2. Prog Nucleic Acid Res Mol Biol. 2002. PMID: 12206451 Review. - Synthesis of vitellogenin, an attractive model for investigating hormone-induced gene activation.
Ryffel GU. Ryffel GU. Mol Cell Endocrinol. 1978 Dec;12(3):237-46. doi: 10.1016/0303-7207(78)90082-5. Mol Cell Endocrinol. 1978. PMID: 367848 Review.
Cited by
- Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes.
Hasan MK, Jeannine Brady L. Hasan MK, et al. J Struct Biol X. 2024 Jun 27;10:100106. doi: 10.1016/j.yjsbx.2024.100106. eCollection 2024 Dec. J Struct Biol X. 2024. PMID: 39040530 Free PMC article. - Autism-Associated Vigilin Depletion Impairs DNA Damage Repair.
Banday S, Pandita RK, Mushtaq A, Bacolla A, Mir US, Singh DK, Jan S, Bhat KP, Hunt CR, Rao G, Charaka VK, Tainer JA, Pandita TK, Altaf M. Banday S, et al. Mol Cell Biol. 2021 Jun 23;41(7):e0008221. doi: 10.1128/MCB.00082-21. Epub 2021 Jun 23. Mol Cell Biol. 2021. PMID: 33941620 Free PMC article. - The KH domain facilitates the substrate specificity and unwinding processivity of DDX43 helicase.
Yadav M, Singh RS, Hogan D, Vidhyasagar V, Yang S, Chung IYW, Kusalik A, Dmitriev OY, Cygler M, Wu Y. Yadav M, et al. J Biol Chem. 2021 Jan-Jun;296:100085. doi: 10.1074/jbc.RA120.015824. Epub 2020 Nov 23. J Biol Chem. 2021. PMID: 33199368 Free PMC article. - Vigilin protein Vgl1 is required for heterochromatin-mediated gene silencing in Schizosaccharomyces pombe.
Farooq Z, Abdullah E, Banday S, Ganai SA, Rashid R, Mushtaq A, Rashid S, Altaf M. Farooq Z, et al. J Biol Chem. 2019 Nov 29;294(48):18029-18040. doi: 10.1074/jbc.RA119.009262. Epub 2019 Sep 25. J Biol Chem. 2019. PMID: 31554660 Free PMC article. - Protein interaction networks at the host-microbe interface in Diaphorina citri, the insect vector of the citrus greening pathogen.
Ramsey JS, Chavez JD, Johnson R, Hosseinzadeh S, Mahoney JE, Mohr JP, Robison F, Zhong X, Hall DG, MacCoss M, Bruce J, Cilia M. Ramsey JS, et al. R Soc Open Sci. 2017 Feb 8;4(2):160545. doi: 10.1098/rsos.160545. eCollection 2017 Feb. R Soc Open Sci. 2017. PMID: 28386418 Free PMC article.