The syntaxin homolog AtPEP12p resides on a late post-Golgi compartment in plants - PubMed (original) (raw)

The syntaxin homolog AtPEP12p resides on a late post-Golgi compartment in plants

A da Silva Conceição et al. Plant Cell. 1997 Apr.

Abstract

Soluble proteins are transported to the plant vacuole through the secretory pathway via membrane-bound vesicles. Targeting of vesicles to appropriate organelles requires several membrane-bound and soluble factors that have been characterized in yeast and mammalian systems. For example, the yeast PEP12 protein is a syntaxin homolog that is involved in protein transport to the yeast vacuole. Previously, we isolated an Arabidopsis thaliana homolog of PEP12 by functional complementation of the yeast pep12 mutant. Antibodies raised against the cytoplasmic portion of AtPEP12 have been prepared and used for intracellular localization of this protein. Biochemical analysis indicates that AtPEP12 does not localize to the endoplasmic reticulum, Golgi apparatus, plasma membrane, or tonoplast in Arabidopsis plants; furthermore, based on biochemical and electron microscopy immunogold labeling analyses, AtPEP12 is likely to be localized to a post-Golgi compartment in the vacuolar pathway.

PubMed Disclaimer

Similar articles

• A putative vacuolar cargo receptor partially colocalizes with AtPEP12p on a prevacuolar compartment in Arabidopsis roots.
  Sanderfoot AA, Ahmed SU, Marty-Mazars D, Rapoport I, Kirchhausen T, Marty F, Raikhel NV. Sanderfoot AA, et al. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9920-5. doi: 10.1073/pnas.95.17.9920. Proc Natl Acad Sci U S A. 1998. PMID: 9707576 Free PMC article.
• An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant.
  Bassham DC, Gal S, da Silva Conceição A, Raikhel NV. Bassham DC, et al. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7262-6. doi: 10.1073/pnas.92.16.7262. Proc Natl Acad Sci U S A. 1995. PMID: 7638178 Free PMC article.
• Overexpression of the Arabidopsis syntaxin PEP12/SYP21 inhibits transport from the prevacuolar compartment to the lytic vacuole in vivo.
  Foresti O, daSilva LL, Denecke J. Foresti O, et al. Plant Cell. 2006 Sep;18(9):2275-93. doi: 10.1105/tpc.105.040279. Epub 2006 Aug 25. Plant Cell. 2006. PMID: 16935987 Free PMC article.
• Protein transport from the late Golgi to the vacuole in the yeast Saccharomyces cerevisiae.
  Bowers K, Stevens TH. Bowers K, et al. Biochim Biophys Acta. 2005 Jul 10;1744(3):438-54. doi: 10.1016/j.bbamcr.2005.04.004. Biochim Biophys Acta. 2005. PMID: 15913810 Review.
• Targeting of tonoplast proteins to the vacuole.
  Rojas-Pierce M. Rojas-Pierce M. Plant Sci. 2013 Oct;211:132-6. doi: 10.1016/j.plantsci.2013.07.005. Epub 2013 Jul 18. Plant Sci. 2013. PMID: 23987818 Review.

Cited by

• Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.
  Dahhan DA, Reynolds GD, Cárdenas JJ, Eeckhout D, Johnson A, Yperman K, Kaufmann WA, Vang N, Yan X, Hwang I, Heese A, De Jaeger G, Friml J, Van Damme D, Pan J, Bednarek SY. Dahhan DA, et al. Plant Cell. 2022 May 24;34(6):2150-2173. doi: 10.1093/plcell/koac071. Plant Cell. 2022. PMID: 35218346 Free PMC article.
• Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell.
  Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV. Sanderfoot AA, et al. Mol Biol Cell. 2001 Dec;12(12):3733-43. doi: 10.1091/mbc.12.12.3733. Mol Biol Cell. 2001. PMID: 11739776 Free PMC article.
• The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidence from competition with its cytosolic domain.
  Geelen D, Leyman B, Batoko H, Di Sansebastiano GP, Moore I, Blatt MR. Geelen D, et al. Plant Cell. 2002 Feb;14(2):387-406. doi: 10.1105/tpc.010328. Plant Cell. 2002. PMID: 11884682 Free PMC article.
• Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins.
  Sohn EJ, Kim ES, Zhao M, Kim SJ, Kim H, Kim YW, Lee YJ, Hillmer S, Sohn U, Jiang L, Hwang I. Sohn EJ, et al. Plant Cell. 2003 May;15(5):1057-70. doi: 10.1105/tpc.009779. Plant Cell. 2003. PMID: 12724533 Free PMC article.
• Mediation of clathrin-dependent trafficking during cytokinesis and cell expansion by Arabidopsis stomatal cytokinesis defective proteins.
  McMichael CM, Reynolds GD, Koch LM, Wang C, Jiang N, Nadeau J, Sack FD, Gelderman MB, Pan J, Bednarek SY. McMichael CM, et al. Plant Cell. 2013 Oct;25(10):3910-25. doi: 10.1105/tpc.113.115162. Epub 2013 Oct 31. Plant Cell. 2013. PMID: 24179130 Free PMC article.

References

1. 1. Histochem J. 1989 Mar;21(3):163-71 - PubMed
2. 1. Anal Biochem. 1996 Oct 1;241(1):140-2 - PubMed
3. 1. Plant Cell. 1995 Jun;7(6):667-676 - PubMed
4. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
5. 1. Science. 1992 Jul 10;257(5067):255-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • PubMed Central

• Molecular Biology Databases

  • Gene Ontology
  • Saccharomyces Genome Database
  • The Arabidopsis Information Resource