CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues - PubMed (original) (raw)

CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues

M Bellorini et al. Nucleic Acids Res. 1997.

Abstract

Both the TATA and CCAAT boxes are widespread promoter elements and their binding proteins, TBP and NF-Y, are extremely conserved in evolution. NF-Y is composed of three subunits, NF-YA, NF-YB and NF-YC, all necessary for DNA binding. NF-YB and NF-YC contain a putative histone-like motif, a domain also present in TBP-associated factors (TAFIIs) and in the subunits of the transcriptional repressor NC2. Immunopurification of holo-TFIID with anti-TBP and anti-TAFII100 antibodies indicates that a fraction of NF-YB associates with TFIID in the absence of NF-YA. Sedimentation velocity centrifugation experiments confirm that two pools of NF-YB, and most likely NF-YC, exist: one associated with NF-YA and binding to the CCAAT box; another involved in high molecular weight complexes. We started to dissect NF-Y-TFIID interactions by showing that: (i) NF-YB and NF-YC interact with TBP in solution, both separately and once bound to each other; (ii) short stretches of both NF-YB and NF-YC located within the evolutionary conserved domains, adjacent to the putative histone fold motifs, are necessary for TBP binding; (iii) TBP single amino acid mutants in the HS2 helix, previously shown to be defective in NC2 binding, are also unable to bind NF-YB and NF-YC.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nucleic Acids Res. 1995 Jul 25;23(14):2685-91 - PubMed
    1. Cell. 1992 Aug 7;70(3):477-89 - PubMed
    1. J Biol Chem. 1995 Sep 8;270(36):20978-86 - PubMed
    1. EMBO J. 1995 Sep 1;14(17):4318-28 - PubMed
    1. J Biol Chem. 1995 Oct 20;270(42):25014-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources