Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning - PubMed (original) (raw)
. 1997 May 1;11(9):1160-8.
doi: 10.1101/gad.11.9.1160.
Affiliations
- PMID: 9159397
- DOI: 10.1101/gad.11.9.1160
Free article
Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning
P Glaser et al. Genes Dev. 1997.
Free article
Abstract
The Bacillus subtilis spo0J gene is required for accurate chromosome partitioning during growth and sporulation. We have characterized the subcellular localization of Spo0J protein by immunofluorescence and, in living cells, by use of a spo0J-gfp fusion. We show that the Spo0J protein forms discrete stable foci usually located close to the cell poles. The foci replicate in concert with the initiation of new rounds of DNA replication, after which the daughter foci migrate apart inside the cell. This migration is independent of cell length extension, and presumably serves to direct the daughter chromosomes toward opposite poles of the cell, ready for division. During sporulation, the foci move to the extreme poles of the cell, where they function to position the oriC region of the chromosome ready for polar septation. These observations provide strong evidence for the existence of a dynamic, mitotic-like apparatus responsible for chromosome partitioning in bacteria.
Similar articles
- RacA, a bacterial protein that anchors chromosomes to the cell poles.
Ben-Yehuda S, Rudner DZ, Losick R. Ben-Yehuda S, et al. Science. 2003 Jan 24;299(5606):532-6. doi: 10.1126/science.1079914. Epub 2002 Dec 19. Science. 2003. PMID: 12493822 - Control of development by altered localization of a transcription factor in B. subtilis.
Quisel JD, Lin DC, Grossman AD. Quisel JD, et al. Mol Cell. 1999 Nov;4(5):665-72. doi: 10.1016/s1097-2765(00)80377-9. Mol Cell. 1999. PMID: 10619014 - Bacterial chromosome segregation.
Draper GC, Gober JW. Draper GC, et al. Annu Rev Microbiol. 2002;56:567-97. doi: 10.1146/annurev.micro.56.012302.160729. Epub 2002 Jan 30. Annu Rev Microbiol. 2002. PMID: 12142478 Review. - Upheaval in the bacterial nucleoid. An active chromosome segregation mechanism.
Sharpe ME, Errington J. Sharpe ME, et al. Trends Genet. 1999 Feb;15(2):70-4. doi: 10.1016/s0168-9525(98)01660-6. Trends Genet. 1999. PMID: 10098410 Review.
Cited by
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.
Tišma M, Kaljević J, Gruber S, Le TBK, Dekker C. Tišma M, et al. FEMS Microbiol Rev. 2024 Jan 12;48(1):fuad067. doi: 10.1093/femsre/fuad067. FEMS Microbiol Rev. 2024. PMID: 38142222 Free PMC article. Review. - Dissecting the roles of peptidoglycan synthetic and autolytic activities in the walled to L-form bacterial transition.
Kawai Y, Errington J. Kawai Y, et al. Front Microbiol. 2023 Jun 2;14:1204979. doi: 10.3389/fmicb.2023.1204979. eCollection 2023. Front Microbiol. 2023. PMID: 37333659 Free PMC article. - Bacillus subtilis, a Swiss Army Knife in Science and Biotechnology.
Stülke J, Grüppen A, Bramkamp M, Pelzer S. Stülke J, et al. J Bacteriol. 2023 May 25;205(5):e0010223. doi: 10.1128/jb.00102-23. Epub 2023 May 4. J Bacteriol. 2023. PMID: 37140386 Free PMC article. Review. - A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis.
Roberts DM. Roberts DM. Microbiologyopen. 2023 Feb;12(1):e1344. doi: 10.1002/mbo3.1344. Microbiologyopen. 2023. PMID: 36825885 Free PMC article. - Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete.
Takacs CN, Wachter J, Xiang Y, Ren Z, Karaboja X, Scott M, Stoner MR, Irnov I, Jannetty N, Rosa PA, Wang X, Jacobs-Wagner C. Takacs CN, et al. Nat Commun. 2022 Nov 22;13(1):7173. doi: 10.1038/s41467-022-34876-4. Nat Commun. 2022. PMID: 36450725 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources