The staining pattern of collagen fibrils. Improved correlation with sequence data - PubMed (original) (raw)
. 1979 Nov 10;254(21):10710-4.
- PMID: 91606
Free article
The staining pattern of collagen fibrils. Improved correlation with sequence data
K M Meek et al. J Biol Chem. 1979.
Free article
Abstract
The periodic banding pattern of stained collagen fibrils observed in the electron microscopic can be correlated with the charge distribution deduced from the amino acid sequence. Earlier work used alpha 1 chain sequence data only. The present study incorporates alpha 2 as well as alpha 1 sequence data, so that the complete distribution of charged residues is used. Correlation is improved if it is supposed that the extrahelical terminal regions are contracted. The optimal value of the periodicity, D, (previously 232.3 +/- 0.5 residues using alpha 1 data only), is now 234.2 +/- 0.5 residues, assuming uniform spacing of residues in the helical body of the molecule. This value agrees better with values obtained by others from analyses of interactions between molecules, using sequence data alone. Using the improved value of D, the relative axial locations of the charged residues in the fibril are displayed. In this way, the charged residues contributing to each band in the fibril staining pattern can be identified.
Similar articles
- A model for type II collagen fibrils: distinctive D-band patterns in native and reconstituted fibrils compared with sequence data for helix and telopeptide domains.
Ortolani F, Giordano M, Marchini M. Ortolani F, et al. Biopolymers. 2000 Nov;54(6):448-63. doi: 10.1002/1097-0282(200011)54:6<448::AID-BIP80>3.0.CO;2-Q. Biopolymers. 2000. PMID: 10951330 - The self-assembly of a mini-fibril with axial periodicity from a designed collagen-mimetic triple helix.
Kaur PJ, Strawn R, Bai H, Xu K, Ordas G, Matsui H, Xu Y. Kaur PJ, et al. J Biol Chem. 2015 Apr 3;290(14):9251-61. doi: 10.1074/jbc.M113.542241. Epub 2015 Feb 11. J Biol Chem. 2015. PMID: 25673694 Free PMC article. - The collagen fibril--a model system for studying the staining and fixation of a protein.
Chapman JA, Tzaphlidou M, Meek KM, Kadler KE. Chapman JA, et al. Electron Microsc Rev. 1990;3(1):143-82. doi: 10.1016/0892-0354(90)90018-n. Electron Microsc Rev. 1990. PMID: 1715773 Review. - Collagen fibril formation.
Kadler KE, Holmes DF, Trotter JA, Chapman JA. Kadler KE, et al. Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):1-11. doi: 10.1042/bj3160001. Biochem J. 1996. PMID: 8645190 Free PMC article. Review.
Cited by
- Crystal-collagen relationships in calcified turkey leg tendons visualized by selected-area dark field electron microscopy.
Arsenault AL. Arsenault AL. Calcif Tissue Int. 1988 Oct;43(4):202-12. doi: 10.1007/BF02555136. Calcif Tissue Int. 1988. PMID: 3145125 - Inorganic-Organic Nanocomposite Assembly Using Collagen as Template and Sodium Tripolyphosphate as A Biomimetic Analog of Matrix Phosphoprotein.
Dai L, Qi YP, Niu LN, Liu Y, Pucci CR, Looney SW, Ling JQ, Pashley DH, Tay FR. Dai L, et al. Cryst Growth Des. 2011 Aug 3;11(8):3504-3511. doi: 10.1021/cg200663v. Cryst Growth Des. 2011. PMID: 21857797 Free PMC article. - The use of glutaraldehyde and tannic acid to preserve reconstituted collagen for electron microscopy.
Meek KM. Meek KM. Histochemistry. 1981;73(1):115-20. doi: 10.1007/BF00493137. Histochemistry. 1981. PMID: 6797991 - Effects of mineralization on the hierarchical organization of collagen-a synchrotron X-ray scattering and polarized second harmonic generation study.
Zheng K, Zhong J, Hu J, Nebbiolo E, Sanchez-Weatherby J, Tang T, Landis WJ, Chen J, Winlove P, Sherlock BE, Bell J. Zheng K, et al. Interface Focus. 2024 Jun 7;14(3):20230046. doi: 10.1098/rsfs.2023.0046. eCollection 2024 Jun. Interface Focus. 2024. PMID: 39081623 Free PMC article. - Using sequence data to predict the self-assembly of supramolecular collagen structures.
Puszkarska AM, Frenkel D, Colwell LJ, Duer MJ. Puszkarska AM, et al. Biophys J. 2022 Aug 16;121(16):3023-3033. doi: 10.1016/j.bpj.2022.07.019. Epub 2022 Jul 20. Biophys J. 2022. PMID: 35859421 Free PMC article.