Propagation of antigen-specific T cell helper function in vitro - PubMed (original) (raw)
. 1979 Dec;123(6):2525-31.
- PMID: 91635
Propagation of antigen-specific T cell helper function in vitro
R D Schrier et al. J Immunol. 1979 Dec.
Abstract
Antigen-induced proliferation of primed lymph node cells was abrogated by treatment with anti-Ly 1 serum and complement (C) but not with anti-Ly 2 serum and C. Lymph node cells from animals primed to ovalbumin were activated with antigen in vitro, followed by propagation in an antigen-free supernatant fluid obtained from lectin-induced normal spleen cells. T cells processed in this manner displayed a stepwise enrichment of helper activity for antibody production as measured in a secondary hapten-carrier response. The sequential increase in antigen-specific help was paralleled by a rise in the antigen-induced proliferative response, a phenomenon whose expression was dependent on the presence of syngeneic or semi-syngeneic irradiated filler cells.