Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane - PubMed (original) (raw)
Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane
U Bömer et al. EMBO J. 1997.
Abstract
The protein transport machinery of the inner mitochondrial membrane contains three essential Tim proteins. Tim17 and Tim23 are thought to build a preprotein translocation channel, while Tim44 transiently interacts with the matrix heat shock protein Hsp70 to form an ATP-driven import motor. For this report we characterized the biogenesis and interactions of Tim proteins. (i) Import of the precursor of Tim44 into the inner membrane requires mtHsp70, whereas import and inner membrane integration of the precursors of Tim17 and Tim23 are independent of functional mtHsp70. (ii) Tim17 efficiently associates with Tim23 and mtHsp70, but only weakly with Tim44. (iii) Depletion of Tim44 does not affect the co-precipitation of Tim17 with antibodies directed against mtHsp70. (iv) Tim23 associates with both Tim44 and Tim17, suggesting the presence of two Tim23 pools in the inner membrane, a Tim44-Tim23-containing sub-complex and a Tim23-Tim17-containing sub-complex. (v) The association of mtHsp70 with the Tim23-Tim17 sub-complex is ATP sensitive and can be distinguished from the mtHsp70-Tim44 interaction by the differential influence of an amino acid substitution in mtHsp70. (vi) Genetic evidence, suppression of the protein import defect of a tim17 yeast mutant by overexpression of mtHsp70 and synthetic lethality of conditional mutants in the genes of Tim17 and mtHsp70, supports a functional interaction of mtHsp70 with Tim17. We conclude that the protein transport machinery of the mitochondrial inner membrane consists of dynamically interacting sub-complexes, each of which transiently binds mtHsp70.
Similar articles
- Mitochondrial protein import: molecular basis of the ATP-dependent interaction of MtHsp70 with Tim44.
Moro F, Okamoto K, Donzeau M, Neupert W, Brunner M. Moro F, et al. J Biol Chem. 2002 Mar 1;277(9):6874-80. doi: 10.1074/jbc.M107935200. Epub 2001 Nov 30. J Biol Chem. 2002. PMID: 11733493 - Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22.
Sirrenberg C, Bauer MF, Guiard B, Neupert W, Brunner M. Sirrenberg C, et al. Nature. 1996 Dec 12;384(6609):582-5. doi: 10.1038/384582a0. Nature. 1996. PMID: 8955274 - The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix.
Moro F, Sirrenberg C, Schneider HC, Neupert W, Brunner M. Moro F, et al. EMBO J. 1999 Jul 1;18(13):3667-75. doi: 10.1093/emboj/18.13.3667. EMBO J. 1999. PMID: 10393182 Free PMC article. - The preprotein translocase of the mitochondrial inner membrane: function and evolution.
Rassow J, Dekker PJ, van Wilpe S, Meijer M, Soll J. Rassow J, et al. J Mol Biol. 1999 Feb 12;286(1):105-20. doi: 10.1006/jmbi.1998.2455. J Mol Biol. 1999. PMID: 9931253 Review. - Molecular chaperones as essential mediators of mitochondrial biogenesis.
Voos W, Röttgers K. Voos W, et al. Biochim Biophys Acta. 2002 Sep 2;1592(1):51-62. doi: 10.1016/s0167-4889(02)00264-1. Biochim Biophys Acta. 2002. PMID: 12191768 Review.
Cited by
- A hybrid TIM complex mediates protein import into hydrogenosomes of Trichomonas vaginalis.
Makki A, Kereïche S, Le T, Kučerová J, Rada P, Žárský V, Hrdý I, Tachezy J. Makki A, et al. BMC Biol. 2024 Jun 3;22(1):130. doi: 10.1186/s12915-024-01928-8. BMC Biol. 2024. PMID: 38825681 Free PMC article. - Tim17 Updates: A Comprehensive Review of an Ancient Mitochondrial Protein Translocator.
Chaudhuri M, Darden C, Gonzalez FS, Singha UK, Quinones L, Tripathi A. Chaudhuri M, et al. Biomolecules. 2020 Dec 7;10(12):1643. doi: 10.3390/biom10121643. Biomolecules. 2020. PMID: 33297490 Free PMC article. Review. - Hsp70 at the membrane: driving protein translocation.
Craig EA. Craig EA. BMC Biol. 2018 Jan 17;16(1):11. doi: 10.1186/s12915-017-0474-3. BMC Biol. 2018. PMID: 29343244 Free PMC article. Review. - Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50.
Malhotra K, Modak A, Nangia S, Daman TH, Gunsel U, Robinson VL, Mokranjac D, May ER, Alder NN. Malhotra K, et al. Sci Adv. 2017 Sep 1;3(9):e1700532. doi: 10.1126/sciadv.1700532. eCollection 2017 Sep. Sci Adv. 2017. PMID: 28879236 Free PMC article. - Disorders of phospholipid metabolism: an emerging class of mitochondrial disease due to defects in nuclear genes.
Lu YW, Claypool SM. Lu YW, et al. Front Genet. 2015 Feb 3;6:3. doi: 10.3389/fgene.2015.00003. eCollection 2015. Front Genet. 2015. PMID: 25691889 Free PMC article. Review.
References
- J Cell Biol. 1994 Dec;127(6 Pt 1):1547-56 - PubMed
- FEBS Lett. 1994 Aug 1;349(2):222-8 - PubMed
- FEBS Lett. 1993 Sep 6;330(1):66-70 - PubMed
- EMBO J. 1992 Dec;11(13):4787-94 - PubMed
- J Biol Chem. 1995 Dec 15;270(50):29848-53 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases