roughex down-regulates G2 cyclins in G1 - PubMed (original) (raw)
. 1997 May 15;11(10):1289-98.
doi: 10.1101/gad.11.10.1289.
Affiliations
- PMID: 9171373
- DOI: 10.1101/gad.11.10.1289
Free article
roughex down-regulates G2 cyclins in G1
B J Thomas et al. Genes Dev. 1997.
Free article
Abstract
Cell cycle arrest in G1 at the onset of patterning in the Drosophila eye is mediated by roughex. In roughex mutants, cells accumulate Cyclin A protein in early G1 and progress into S phase precociously. When Roughex is overexpressed in S/G2 cells, Cyclin A is mislocalized to the nucleus and degraded, preventing mitosis. Whereas Roughex inhibits Cyclin A accumulation, Cyclin E down-regulates Roughex protein in vivo. Roughex binds to Cyclin E and is a substrate for a Cyclin E-Cdk complex in vitro. These data argue that Roughex inhibits Cyclin A accumulation in early G1 by targeting Cyclin A for destruction. In late G1, Roughex is destabilized in a Cyclin E-dependent process, releasing Cyclin A for its role in S/G2.
Similar articles
- Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A.
Dong X, Zavitz KH, Thomas BJ, Lin M, Campbell S, Zipursky SL. Dong X, et al. Genes Dev. 1997 Jan 1;11(1):94-105. doi: 10.1101/gad.11.1.94. Genes Dev. 1997. PMID: 9000053 - Roughex mediates G(1) arrest through a physical association with cyclin A.
Avedisov SN, Krasnoselskaya I, Mortin M, Thomas BJ. Avedisov SN, et al. Mol Cell Biol. 2000 Nov;20(21):8220-9. doi: 10.1128/MCB.20.21.8220-8229.2000. Mol Cell Biol. 2000. PMID: 11027291 Free PMC article. - Sister chromatids fail to separate during an induced endoreplication cycle in Drosophila embryos.
Vidwans SJ, DiGregorio PJ, Shermoen AW, Foat B, Iwasa J, Yakubovich N, O'Farrell PH. Vidwans SJ, et al. Curr Biol. 2002 May 14;12(10):829-33. doi: 10.1016/s0960-9822(02)00845-x. Curr Biol. 2002. PMID: 12015119 Free PMC article. - [Cell cycle regulation after exposure to ionizing radiation].
Teyssier F, Bay JO, Dionet C, Verrelle P. Teyssier F, et al. Bull Cancer. 1999 Apr;86(4):345-57. Bull Cancer. 1999. PMID: 10341340 Review. French. - Cytometry of cyclin proteins.
Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Darzynkiewicz Z, et al. Cytometry. 1996 Sep 1;25(1):1-13. doi: 10.1002/(SICI)1097-0320(19960901)25:1<1::AID-CYTO1>3.0.CO;2-N. Cytometry. 1996. PMID: 8875049 Review.
Cited by
- Pattern reorganization occurs independently of cell division during Drosophila wing disc regeneration in situ.
Díaz-García S, Baonza A. Díaz-García S, et al. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13032-7. doi: 10.1073/pnas.1220543110. Epub 2013 Jul 22. Proc Natl Acad Sci U S A. 2013. PMID: 23878228 Free PMC article. - A genetic screen for dominant modifiers of a cyclin E hypomorphic mutation identifies novel regulators of S-phase entry in Drosophila.
Brumby A, Secombe J, Horsfield J, Coombe M, Amin N, Coates D, Saint R, Richardson H. Brumby A, et al. Genetics. 2004 Sep;168(1):227-51. doi: 10.1534/genetics.104.026617. Genetics. 2004. PMID: 15454540 Free PMC article. - "Mitotic" kinesin-5 is a dynamic brake for axonal growth.
Lu W, Lee BS, Deng HXY, Lakonishok M, Martin-Blanco E, Gelfand VI. Lu W, et al. bioRxiv [Preprint]. 2024 Sep 15:2024.09.12.612721. doi: 10.1101/2024.09.12.612721. bioRxiv. 2024. PMID: 39314406 Free PMC article. Preprint. - A population of G2-arrested cells are selected as sensory organ precursors for the interommatidial bristles of the Drosophila eye.
Meserve JH, Duronio RJ. Meserve JH, et al. Dev Biol. 2017 Oct 15;430(2):374-384. doi: 10.1016/j.ydbio.2017.06.023. Epub 2017 Jun 21. Dev Biol. 2017. PMID: 28645749 Free PMC article. - Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways.
Read RD, Bach EA, Cagan RL. Read RD, et al. Mol Cell Biol. 2004 Aug;24(15):6676-89. doi: 10.1128/MCB.24.15.6676-6689.2004. Mol Cell Biol. 2004. PMID: 15254235 Free PMC article.