Cellular characteristics of peripheral blood lymphocytes and tumour-infiltrating lymphocytes in patients with gynaecological tumours - PubMed (original) (raw)
Comparative Study
Cellular characteristics of peripheral blood lymphocytes and tumour-infiltrating lymphocytes in patients with gynaecological tumours
T Schöndorf et al. Cancer Immunol Immunother. 1997 Apr.
Abstract
Immunotherapy of gynaecological cancer with tumour-infiltrating lymphocytes (TIL) or peripheral blood lymphocytes (PBL) has become a valid treatment modality with varying degrees of success in obtaining an antitumour response. TIL consist of lymphocytes, mainly T cells and minor populations of natural killer cells or B cells. Conventional cytogenetic studies of tumour cells from patients with breast and ovarian cancer have shown multiple chromosomal abnormalities including chromosomes 7 and 12. This study was designed to analyse the surface further, as well as investigate the intracellular, characteristics of TIL by multicolour flow cytometry and the cytogenetic features by fluorescence in situ hybridization. Tumour cell, peripheral blood and TIL samples from 25 patients (15 ovarian tumours, 8 breast cancers, 1 uterine sarcoma, 1 cervical carcinoma) were analysed for their phenotype, the expression of major cytokines [interleukin-2 (IL-2), IL-4 and interferon gamma (IFN gamma)], their proliferation rate, their cytotoxic ability and for the presence of numerical aberrations of chromosomes 7 and 12. All the tumour cells showed a high frequency of numerical aberration in chromosomes 7 and 12, especially trisomies or tetrasomies and combined aberrations. Trisomies of both chromosomes also occured at a low percentage in TIL and PBL. The phenotyping of TIL and PBL revealed rather similar subsets of lymphocytes. In both, T cells were the major population, with TIL containing a slightly increased CD4/CD8 ratio. The cytokine pattern showed a predominance of IL-4 production in TIL and of IFN gamma in PBL, indicating that, in TIL, cellular immunity is downregulated, whereas in PBL the cytotoxic immune response predominates. This is in accordance with the cytotoxic ability of TIL, which is weakened in comparison to PBL. Cellular characteristics revealed some disadvantages in the use of TIL for cancer treatment, explaining ineffective clinical results. The search for specific antitumour lymphocytes requires carefully designed experiments in order to define effective anticancer cells and thereby improve immunologically mediated tumour therapy.
Similar articles
- Phenotypic and functional analysis of tumor-infiltrating lymphocytes compared with tumor-associated lymphocytes from ascitic fluid and peripheral blood lymphocytes in patients with advanced ovarian cancer.
Santin AD, Hermonat PL, Ravaggi A, Bellone S, Roman JJ, Smith CV, Pecorelli S, Radominska-Pandya A, Cannon MJ, Parham GP. Santin AD, et al. Gynecol Obstet Invest. 2001;51(4):254-61. doi: 10.1159/000058060. Gynecol Obstet Invest. 2001. PMID: 11408737 - Phenotype, cytokine production and cytolytic capacity of fresh (uncultured) tumour-infiltrating T lymphocytes in human renal cell carcinoma.
Van den Hove LE, Van Gool SW, Van Poppel H, Baert L, Coorevits L, Van Damme B, Ceuppens JL. Van den Hove LE, et al. Clin Exp Immunol. 1997 Sep;109(3):501-9. doi: 10.1046/j.1365-2249.1997.4771375.x. Clin Exp Immunol. 1997. PMID: 9328129 Free PMC article. - Clinical significance of tumor-infiltrating lymphocytes in breast cancer.
Stanton SE, Disis ML. Stanton SE, et al. J Immunother Cancer. 2016 Oct 18;4:59. doi: 10.1186/s40425-016-0165-6. eCollection 2016. J Immunother Cancer. 2016. PMID: 27777769 Free PMC article. Review. - Emerging interleukin targets in the tumour microenvironment: implications for the treatment of gastrointestinal tumours.
Dickerson LK, Carter JA, Kohli K, Pillarisetty VG. Dickerson LK, et al. Gut. 2023 Aug;72(8):1592-1606. doi: 10.1136/gutjnl-2023-329650. Epub 2023 May 31. Gut. 2023. PMID: 37258094 Review.
Cited by
- Tumor‑infiltrating CD8+ T cells as a biomarker for chemotherapy efficacy in unresectable hepatocellular carcinoma.
Kuwano A, Yada M, Miyazaki Y, Tanaka K, Kurosaka K, Ohishi Y, Masumoto A, Motomura K. Kuwano A, et al. Oncol Lett. 2023 Apr 28;25(6):259. doi: 10.3892/ol.2023.13845. eCollection 2023 Jun. Oncol Lett. 2023. PMID: 37205921 Free PMC article. - Impact of CyberKnife Radiosurgery on Overall Survival and Various Parameters of Patients with 1-3 versus ≥ 4 Brain Metastases.
Murovic J, Ding V, Han SS, Adler JR, Chang SD. Murovic J, et al. Cureus. 2017 Oct 24;9(10):e1798. doi: 10.7759/cureus.1798. Cureus. 2017. PMID: 29282442 Free PMC article. - CD8+ lymphocyte infiltration is an independent favorable prognostic indicator in basal-like breast cancer.
Liu S, Lachapelle J, Leung S, Gao D, Foulkes WD, Nielsen TO. Liu S, et al. Breast Cancer Res. 2012 Mar 15;14(2):R48. doi: 10.1186/bcr3148. Breast Cancer Res. 2012. PMID: 22420471 Free PMC article. Clinical Trial. - Maturation of circulating dendritic cells and imbalance of T-cell subsets in patients with squamous cell carcinoma of the head and neck.
Sakakura K, Chikamatsu K, Takahashi K, Whiteside TL, Furuya N. Sakakura K, et al. Cancer Immunol Immunother. 2006 Feb;55(2):151-9. doi: 10.1007/s00262-005-0697-y. Epub 2005 May 12. Cancer Immunol Immunother. 2006. PMID: 15889251 Free PMC article. - Prognostic value of CD4+ lymphocytes in pleural cavity of patients with non-small cell lung cancer.
Takahashi K, Saito S, Kamamura Y, Katakawa M, Monden Y. Takahashi K, et al. Thorax. 2001 Aug;56(8):639-42. doi: 10.1136/thorax.56.8.639. Thorax. 2001. PMID: 11462067 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials