Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile - PubMed (original) (raw)

Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile

P Marteau et al. J Dairy Sci. 1997 Jun.

Free article

Abstract

This study was conducted to validate a dynamic model of the stomach and small intestine to quantify the survival of lactic acid bacteria and to assess the influence of gastrointestinal secretions. The survival of a single strain of each of the following species, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus, was measured under physiological conditions (e.g., peristalsis, changes in pH, and changes in concentrations of enzymes and bile) and were compared with data obtained from humans. No significant differences were found between the in vitro and in vivo data, indicating that the model has a predictive value for the survival of these bacteria in humans. The survival of these strains of lactic acid bacteria in the gastrointestinal model was investigated under two different conditions in the small intestine: simulation of physiological secretion of bile and low bile secretion. Reductions in viability were significantly different between the bacterial species. The dose-response effect of bile on the survival of the tested bacteria was significant, demonstrating the bactericidal effect of bile salts. This study demonstrates the differences among bacterial species in their sensitivity to gastric and intestinal secretions.

PubMed Disclaimer

Cited by

Congruent strain specific intestinal persistence of Lactobacillus plantarum in an intestine-mimicking in vitro system and in human volunteers.
van Bokhorst-van de Veen H, van Swam I, Wels M, Bron PA, Kleerebezem M. van Bokhorst-van de Veen H, et al. PLoS One. 2012;7(9):e44588. doi: 10.1371/journal.pone.0044588. Epub 2012 Sep 6. PLoS One. 2012. PMID: 22970257 Free PMC article.
Compatibility, Cytotoxicity, and Gastrointestinal Tenacity of Bacteriocin-Producing Bacteria Selected for a Consortium Probiotic Formulation to Be Used in Livestock Feed.
Eveno M, Savard P, Belguesmia Y, Bazinet L, Gancel F, Drider D, Fliss I. Eveno M, et al. Probiotics Antimicrob Proteins. 2021 Feb;13(1):208-217. doi: 10.1007/s12602-020-09687-y. Probiotics Antimicrob Proteins. 2021. PMID: 32712896
Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit.
Ceuppens S, Uyttendaele M, Drieskens K, Heyndrickx M, Rajkovic A, Boon N, Van de Wiele T. Ceuppens S, et al. Appl Environ Microbiol. 2012 Nov;78(21):7698-705. doi: 10.1128/AEM.02142-12. Epub 2012 Aug 24. Appl Environ Microbiol. 2012. PMID: 22923409 Free PMC article.
Long-Term Use of Probiotic-Containing Yogurts Is a Safe Way to Prevent Helicobacter pylori: Based on a Mongolian Gerbil's Model.
Kuo CH, Wang SS, Lu CY, Hu HM, Kuo FC, Weng BC, Wu CC, Liu CJ, Tsai PY, Lee TC, Chen LW, Cheng KH, Chang LL, Wu DC. Kuo CH, et al. Biochem Res Int. 2013;2013:594561. doi: 10.1155/2013/594561. Epub 2013 Nov 21. Biochem Res Int. 2013. PMID: 24349780 Free PMC article.
Role of the gut in modulating lipoprotein metabolism.
Hennessy AA, Ross RP, Fitzgerald GF, Caplice N, Stanton C. Hennessy AA, et al. Curr Cardiol Rep. 2014 Aug;16(8):515. doi: 10.1007/s11886-014-0515-2. Curr Cardiol Rep. 2014. PMID: 24950675 Review.

Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile - PubMed (original) (raw)