Infection of the germ line by retroviral particles produced in the follicle cells: a possible mechanism for the mobilization of the gypsy retroelement of Drosophila - PubMed (original) (raw)
Infection of the germ line by retroviral particles produced in the follicle cells: a possible mechanism for the mobilization of the gypsy retroelement of Drosophila
S U Song et al. Development. 1997 Jul.
Abstract
The gypsy retroelement of Drosophila moves at high frequency in the germ line of the progeny of females carrying a mutation in the flamenco (flam) gene. This high rate of de novo insertion correlates with elevated accumulation of full-length gypsy RNA in the ovaries of these females, as well as the presence of an env-specific RNA. We have prepared monoclonal antibodies against the gypsy Pol and Env products and found that these proteins are expressed in the ovaries of flam females and processed in the manner characteristic of vertebrate retroviruses. The Pol proteins are expressed in both follicle and nurse cells, but they do not accumulate at detectable levels in the oocyte. The Env proteins are expressed exclusively in the follicle cells starting at stage 9 of oogenesis, where they accumulate in the secretory apparatus of the endoplasmic reticulum. They then migrate to the inner side of the cytoplasmic membrane where they assemble into viral particles. These particles can be observed in the perivitelline space starting at stage 10 by immunoelectron microscopy using anti-Env antibodies. We propose a model to explain flamenco-mediated induction of gypsy mobilization that involves the synthesis of gypsy viral particles in the follicle cells, from where they leave and infect the oocyte, thus explaining gypsy insertion into the germ line of the subsequent generation.
Similar articles
- An env-like protein encoded by a Drosophila retroelement: evidence that gypsy is an infectious retrovirus.
Song SU, Gerasimova T, Kurkulos M, Boeke JD, Corces VG. Song SU, et al. Genes Dev. 1994 Sep 1;8(17):2046-57. doi: 10.1101/gad.8.17.2046. Genes Dev. 1994. PMID: 7958877 - Life cycle of an endogenous retrovirus, ZAM, in Drosophila melanogaster.
Leblanc P, Desset S, Giorgi F, Taddei AR, Fausto AM, Mazzini M, Dastugue B, Vaury C. Leblanc P, et al. J Virol. 2000 Nov;74(22):10658-69. doi: 10.1128/jvi.74.22.10658-10669.2000. J Virol. 2000. PMID: 11044110 Free PMC article. - Expression of the Drosophila retrovirus gypsy as ultrastructurally detectable particles in the ovaries of flies carrying a permissive flamenco allele.
Lécher P, Bucheton A, Pélisson A. Lécher P, et al. J Gen Virol. 1997 Sep;78 ( Pt 9):2379-88. doi: 10.1099/0022-1317-78-9-2379. J Gen Virol. 1997. PMID: 9292028 - The relationship between the flamenco gene and gypsy in Drosophila: how to tame a retrovirus.
Bucheton A. Bucheton A. Trends Genet. 1995 Sep;11(9):349-53. doi: 10.1016/s0168-9525(00)89105-2. Trends Genet. 1995. PMID: 7482786 Review. - Drosophila germline invasion by the endogenous retrovirus gypsy: involvement of the viral env gene.
Pelisson A, Mejlumian L, Robert V, Terzian C, Bucheton A. Pelisson A, et al. Insect Biochem Mol Biol. 2002 Oct;32(10):1249-56. doi: 10.1016/s0965-1748(02)00088-7. Insect Biochem Mol Biol. 2002. PMID: 12225916 Review.
Cited by
- piRNA-Guided Transposon Silencing and Response to Stress in Drosophila Germline.
Ho S, Theurkauf W, Rice N. Ho S, et al. Viruses. 2024 Apr 30;16(5):714. doi: 10.3390/v16050714. Viruses. 2024. PMID: 38793595 Free PMC article. Review. - Unistrand piRNA clusters are an evolutionarily conserved mechanism to suppress endogenous retroviruses across the Drosophila genus.
van Lopik J, Alizada A, Trapotsi MA, Hannon GJ, Bornelöv S, Czech Nicholson B. van Lopik J, et al. Nat Commun. 2023 Nov 13;14(1):7337. doi: 10.1038/s41467-023-42787-1. Nat Commun. 2023. PMID: 37957172 Free PMC article. - The composition of piRNA clusters in Drosophila melanogaster deviates from expectations under the trap model.
Wierzbicki F, Kofler R. Wierzbicki F, et al. BMC Biol. 2023 Oct 20;21(1):224. doi: 10.1186/s12915-023-01727-7. BMC Biol. 2023. PMID: 37858221 Free PMC article. - Reactivation of a somatic errantivirus and germline invasion in Drosophila ovaries.
Yoth M, Maupetit-Méhouas S, Akkouche A, Gueguen N, Bertin B, Jensen S, Brasset E. Yoth M, et al. Nat Commun. 2023 Sep 29;14(1):6096. doi: 10.1038/s41467-023-41733-5. Nat Commun. 2023. PMID: 37773253 Free PMC article. - Ongoing endeavors to detect mobilization of transposable elements.
Lee Y, Ha U, Moon S. Lee Y, et al. BMB Rep. 2022 Jul;55(7):305-315. doi: 10.5483/BMBRep.2022.55.7.088. BMB Rep. 2022. PMID: 35725016 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases