Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type 1 inositol 1,4,5-trisphosphate receptor gene - PubMed (original) (raw)
Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type 1 inositol 1,4,5-trisphosphate receptor gene
Y Konishi et al. J Neurochem. 1997 Aug.
Free article
Abstract
The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -398 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E-box consensus sequence. Electrophoretic mobility shift assay revealed CNS-related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay and competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.
Similar articles
- Activation of the mouse inositol 1,4,5-trisphosphate receptor type 1 promoter by AP-2.
Ohkawa N, Konishi Y, Shimada M, Makino Y, Yoshikawa S, Mikoshiba K, Tamura T. Ohkawa N, et al. Gene. 1999 Mar 18;229(1-2):11-9. doi: 10.1016/s0378-1119(99)00048-7. Gene. 1999. PMID: 10095099 - Transcriptional regulation of mouse type 1 inositol 1,4,5-trisphosphate receptor gene by NeuroD-related factor.
Konishi Y, Ohkawa N, Makino Y, Ohkubo H, Kageyama R, Furuichi T, Mikoshiba K, Tamura T. Konishi Y, et al. J Neurochem. 1999 Apr;72(4):1717-24. doi: 10.1046/j.1471-4159.1999.721717.x. J Neurochem. 1999. PMID: 10098882 - Transcription initiation sites and promoter structure of the mouse type 2 inositol 1,4,5-trisphosphate receptor gene.
Morikawa K, Ohbayashi T, Nakagawa M, Konishi Y, Makino Y, Yamada M, Miyawaki A, Furuichi T, Mikoshiba K, Tamura T. Morikawa K, et al. Gene. 1997 Sep 1;196(1-2):181-5. doi: 10.1016/s0378-1119(97)00224-2. Gene. 1997. PMID: 9322756 - Promoter structure and gene expression of the mouse inositol 1,4,5-trisphosphate receptor type 3 gene.
Tamura T, Hashimoto M, Aruga J, Konishi Y, Nakagawa M, Ohbayashi T, Shimada M, Mikoshiba K. Tamura T, et al. Gene. 2001 Sep 5;275(1):169-76. doi: 10.1016/s0378-1119(01)00658-8. Gene. 2001. PMID: 11574166 - Regulation of gene expression by nuclear hormone receptors.
Ham J, Parker MG. Ham J, et al. Curr Opin Cell Biol. 1989 Jun;1(3):503-11. doi: 10.1016/0955-0674(89)90012-4. Curr Opin Cell Biol. 1989. PMID: 2560655 Review. No abstract available.
Cited by
- CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors.
Arige V, Terry LE, Malik S, Knebel TR, Wagner Ii LE, Yule DI. Arige V, et al. J Cell Sci. 2021 Oct 15;134(20):jcs258875. doi: 10.1242/jcs.258875. Epub 2021 Oct 26. J Cell Sci. 2021. PMID: 34533188 Free PMC article. - Skeletal muscle IP3R1 receptors amplify physiological and pathological synaptic calcium signals.
Zhu H, Bhattacharyya BJ, Lin H, Gomez CM. Zhu H, et al. J Neurosci. 2011 Oct 26;31(43):15269-83. doi: 10.1523/JNEUROSCI.3766-11.2011. J Neurosci. 2011. PMID: 22031873 Free PMC article. - Tumor necrosis factor-alpha-mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter.
Park KM, Yule DI, Bowers WJ. Park KM, et al. J Biol Chem. 2009 Oct 2;284(40):27557-66. doi: 10.1074/jbc.M109.034504. Epub 2009 Aug 7. J Biol Chem. 2009. PMID: 19666470 Free PMC article. - Identification of the C-terminal activation domain of the NeuroD-related factor (NDRF).
Konishi Y, Aoki T, Ohkawa N, Matsu-Ura T, Mikoshiba K, Tamura T. Konishi Y, et al. Nucleic Acids Res. 2000 Jun 15;28(12):2406-12. doi: 10.1093/nar/28.12.2406. Nucleic Acids Res. 2000. PMID: 10871374 Free PMC article. - Calcineurin controls inositol 1,4,5-trisphosphate type 1 receptor expression in neurons.
Genazzani AA, Carafoli E, Guerini D. Genazzani AA, et al. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5797-801. doi: 10.1073/pnas.96.10.5797. Proc Natl Acad Sci U S A. 1999. PMID: 10318964 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases