Genetic redundancy between SPT23 and MGA2: regulators of Ty-induced mutations and Ty1 transcription in Saccharomyces cerevisiae - PubMed (original) (raw)

Genetic redundancy between SPT23 and MGA2: regulators of Ty-induced mutations and Ty1 transcription in Saccharomyces cerevisiae

S Zhang et al. Mol Cell Biol. 1997 Aug.

Abstract

SPT23 was isolated as a dosage-dependent suppressor of Ty-induced mutations in Saccharomyces cerevisiae. SPT23 shows considerable sequence homology with MGA2, a gene identified as a dosage-dependent suppressor of a snf2-imposed block on STA1 transcription in S. cerevisiae var. diastaticus. Although single mutations in either of these genes have only modest effects on cell growth, spt23 mga2 double mutants are inviable. Unlike SPT23, multicopy expression of a truncated form of MGA2 suppresses a narrow subset of Ty-induced mutations. SPT23/MGA2 and the SNF/SWI genes affect transcription of certain target genes in similar ways. Spt23p appears to be a rate-limiting component required for functional HIS4 expression of his4-912delta, a promoter insertion mutation induced by the Ty1-912 long terminal repeat. Furthermore, both Spt23p and Mga2p can activate transcription when fused to the Gal4p DNA-binding domain, as previously observed with Snf2p and Snf5p. A 50-amino-acid region in the N terminus of the predicted Spt23p protein is necessary and sufficient for the transactivation and necessary for suppression of Ty1-induced mutations and the essential function of Spt23p. Cell fractionation and cytological experiments suggest that Spt23p is associated with the nucleus. Our results suggest that SPT23/MGA2 affects transcription of a subset of genes in yeast, perhaps by changing chromatin accessibility.

PubMed Disclaimer

Cited by

MGA2 and SPT23 are modifiers of transcriptional silencing in yeast.
Dula ML, Holmes SG. Dula ML, et al. Genetics. 2000 Nov;156(3):933-41. doi: 10.1093/genetics/156.3.933. Genetics. 2000. PMID: 11063674 Free PMC article.
The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation.
Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA. Hitchcock AL, et al. Mol Biol Cell. 2001 Oct;12(10):3226-41. doi: 10.1091/mbc.12.10.3226. Mol Biol Cell. 2001. PMID: 11598205 Free PMC article.
Mitofusin-mediated contacts between mitochondria and peroxisomes regulate mitochondrial fusion.
Alsayyah C, Singh MK, Morcillo-Parra MA, Cavellini L, Shai N, Schmitt C, Schuldiner M, Zalckvar E, Mallet A, Belgareh-Touzé N, Zimmer C, Cohen MM. Alsayyah C, et al. PLoS Biol. 2024 Apr 26;22(4):e3002602. doi: 10.1371/journal.pbio.3002602. eCollection 2024 Apr. PLoS Biol. 2024. PMID: 38669296 Free PMC article.
Transcriptional regulation of desaturase genes in Pichia pastoris GS115.
Yu AQ, Shi TL, Zhang B, Xing LJ, Li MC. Yu AQ, et al. Lipids. 2012 Nov;47(11):1099-108. doi: 10.1007/s11745-012-3712-z. Epub 2012 Sep 8. Lipids. 2012. PMID: 22961009

References

1. Nucleic Acids Res. 1992 Mar 25;20(6):1425 - PubMed
1. Genetics. 1991 May;128(1):69-77 - PubMed
1. Genes Dev. 1992 Jul;6(7):1319-31 - PubMed
1. Genes Dev. 1992 Sep;6(9):1707-15 - PubMed
1. Genetics. 1992 Oct;132(2):325-36 - PubMed

Genetic redundancy between SPT23 and MGA2: regulators of Ty-induced mutations and Ty1 transcription in Saccharomyces cerevisiae - PubMed (original) (raw)