Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro - PubMed (original) (raw)
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Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro
J C Marinoni et al. Methods. 1997 Jul.
Abstract
We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.
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