In vitro analysis of the stop-transfer process during translocation across the cytoplasmic membrane of Escherichia coli - PubMed (original) (raw)
. 1997 Aug 8;272(32):20082-7.
doi: 10.1074/jbc.272.32.20082.
Affiliations
- PMID: 9242681
- DOI: 10.1074/jbc.272.32.20082
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In vitro analysis of the stop-transfer process during translocation across the cytoplasmic membrane of Escherichia coli
K Sato et al. J Biol Chem. 1997.
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Abstract
In this study, using a derivative of proOmpA containing an artificial stop-transfer sequence (proOmpA2xH1), we analyzed the process of stop-transfer during translocation across the cytoplasmic membrane of Escherichia coli. ProOmpA2xH1 did not interfere with the transit of wild-type proOmpA. When proOmpA2xH1 was anchored in the membrane, membrane-inserted SecA was deinserted with the reversion of the inverted topology of SecG. Cross-linking experiments revealed that the anchored proOmpA2xH1 that does not interact with either SecY or SecA. These results, taken together, suggest that proOmpA2xH1 leaves the translocation pathway by means of a specific interaction between the stop-transfer sequence and the translocational channel.
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