Pore formation and translocation of melittin - PubMed (original) (raw)
Pore formation and translocation of melittin
K Matsuzaki et al. Biophys J. 1997 Aug.
Abstract
Melittin, a bee venom, is a basic amphiphilic peptide, which mainly acts on the lipid matrix of membranes, lysing various cells. To elucidate the molecular mechanism, we investigated its interactions with phospholipid vesicles. The peptide formed a pore with a short lifetime in the membrane, as revealed by the release of an anionic fluorescent dye, calcein, from the liposomes. Our new double-labeling method clarified that the pore size increased with the peptide-to-lipid ratio. Upon the disintegration of the pore, a fraction of the peptides translocated across the bilayer. The pore formation was coupled with the translocation, which was proved by three fluorescence experiments recently developed by our laboratory. A novel model for the melittin pore formation was discussed in comparison with other pore-forming peptides.
Similar articles
- Pore formation induced by the peptide melittin in different lipid vesicle membranes.
Rex S. Rex S. Biophys Chem. 1996 Jan 16;58(1-2):75-85. doi: 10.1016/0301-4622(95)00087-9. Biophys Chem. 1996. PMID: 8679920 - Quantitative studies on the melittin-induced leakage mechanism of lipid vesicles.
Rex S, Schwarz G. Rex S, et al. Biochemistry. 1998 Feb 24;37(8):2336-45. doi: 10.1021/bi971009p. Biochemistry. 1998. PMID: 9485380 - Nucleation and growth of pores in 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) / cholesterol bilayer by antimicrobial peptides melittin, its mutants and cecropin P1.
Lyu Y, Fitriyanti M, Narsimhan G. Lyu Y, et al. Colloids Surf B Biointerfaces. 2019 Jan 1;173:121-127. doi: 10.1016/j.colsurfb.2018.09.049. Epub 2018 Sep 24. Colloids Surf B Biointerfaces. 2019. PMID: 30278360 - Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: pore formation by melittin.
Ladokhin AS, Selsted ME, White SH. Ladokhin AS, et al. Biophys J. 1997 Apr;72(4):1762-6. doi: 10.1016/S0006-3495(97)78822-2. Biophys J. 1997. PMID: 9083680 Free PMC article. - Release of lipid vesicle contents by an antibacterial cecropin A-melittin hybrid peptide.
Mancheño JM, Oñaderra M, Martínez del Pozo A, Díaz-Achirica P, Andreu D, Rivas L, Gavilanes JG. Mancheño JM, et al. Biochemistry. 1996 Jul 30;35(30):9892-9. doi: 10.1021/bi953058c. Biochemistry. 1996. PMID: 8703963
Cited by
- Measuring melittin uptake into hydrogel nanoparticles with near-infrared single nanoparticle surface plasmon resonance microscopy.
Cho K, Fasoli JB, Yoshimatsu K, Shea KJ, Corn RM. Cho K, et al. Anal Chem. 2015;87(9):4973-9. doi: 10.1021/acs.analchem.5b00776. Epub 2015 Apr 14. Anal Chem. 2015. PMID: 25844641 Free PMC article. - Non-metabolic membrane tubulation and permeability induced by bioactive peptides.
Lamazière A, Burlina F, Wolf C, Chassaing G, Trugnan G, Ayala-Sanmartin J. Lamazière A, et al. PLoS One. 2007 Feb 14;2(2):e201. doi: 10.1371/journal.pone.0000201. PLoS One. 2007. PMID: 17299584 Free PMC article. - Antimicrobial peptides in toroidal and cylindrical pores.
Mihajlovic M, Lazaridis T. Mihajlovic M, et al. Biochim Biophys Acta. 2010 Aug;1798(8):1485-93. doi: 10.1016/j.bbamem.2010.04.004. Epub 2010 Apr 18. Biochim Biophys Acta. 2010. PMID: 20403332 Free PMC article. - Mechanistic Landscape of Membrane-Permeabilizing Peptides.
Guha S, Ghimire J, Wu E, Wimley WC. Guha S, et al. Chem Rev. 2019 May 8;119(9):6040-6085. doi: 10.1021/acs.chemrev.8b00520. Epub 2019 Jan 9. Chem Rev. 2019. PMID: 30624911 Free PMC article. Review. - Interaction of the gelsolin-derived antibacterial PBP 10 peptide with lipid bilayers and cell membranes.
Bucki R, Janmey PA. Bucki R, et al. Antimicrob Agents Chemother. 2006 Sep;50(9):2932-40. doi: 10.1128/AAC.00134-06. Antimicrob Agents Chemother. 2006. PMID: 16940084 Free PMC article.
References
- Biochim Biophys Acta. 1992 Sep 21;1110(1):97-104 - PubMed
- Biophys J. 1996 Nov;71(5):2669-79 - PubMed
- Biochemistry. 1995 May 16;34(19):6521-6 - PubMed
- Biochim Biophys Acta. 1995 Oct 4;1239(1):51-7 - PubMed
- J Biol Chem. 1982 Mar 10;257(5):2469-76 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources