Characterization of a putative new HPV genomic sequence from a cervical lesion using L1 consensus primers and restriction fragment length polymorphism - PubMed (original) (raw)
Characterization of a putative new HPV genomic sequence from a cervical lesion using L1 consensus primers and restriction fragment length polymorphism
G Astori et al. Virus Res. 1997 Jul.
Abstract
Various methods have been proposed for HPV detection and typing. Prevalence and distribution among types have varied depending upon the methods used and the populations studied. We have applied the polymerase chain reaction (PCR) followed by a Restriction Fragment Length Polymorphism (RFLP) analysis using the MY09/MY11 primers for detection of HPV in cervicovaginal lavages obtained from 323 patients who were referred to our Clinical Department either for genital complaints or an abnormal PAP smear. We assessed (i) the prevalence of HPV and (ii) the reliability of RFLP-typing. For the latter, 35 PCR-HPV products were sequenced. HPV-DNA was detected in 40/197 (20.3%) patients with normal cytology 86/111 (77.5%) with LSIL and 11/15 (73.3%) with HSIL. HPV-16 was the most common type detected in normal cervical cytology samples (10/40, 25%), whereas HPV 16 and 18 were detected in 36/97 (37.1%) of the LSIL and HSIL patients, evidencing the presence of these high-risk HPV types not only in malignant conditions. Results obtained after partial nucleotide sequencing confirmed the results obtained by RFLP analysis. In this study, a putative new HPV fragment (GA6053) was identified. Its closest homology to other known HPV types is 73.8% to HPV-62, 73.0% to HPV-61 and 67.7% to HPV-18. The use of degenerate primers, in conjunction with RFLP, proved to be a reliable method for HPV detection and typing.
Similar articles
- Detection and typing of human papillomaviruses combining different methods: polymerase chain reaction, restriction fragment length polymorphism, line probe assay and sequencing.
Milutin Gasperov N, Sabol I, Matovina M, Spaventi S, Grce M. Milutin Gasperov N, et al. Pathol Oncol Res. 2008 Dec;14(4):355-63. doi: 10.1007/s12253-008-9084-2. Epub 2008 Aug 28. Pathol Oncol Res. 2008. PMID: 18752054 - Detection and typing of human papillomavirus DNA by PCR using consensus primers in various cervical lesions of Korean women.
Hwang T. Hwang T. J Korean Med Sci. 1999 Dec;14(6):593-9. doi: 10.3346/jkms.1999.14.6.593. J Korean Med Sci. 1999. PMID: 10642935 Free PMC article. Clinical Trial. - Variants of human papillomavirus types 53, 58 and 66 identified in Central Brazil.
Cerqueira DM, Camara GN, da Cruz MR, Silva EO, Brígido Mde M, Carvalho LG, Martins CR. Cerqueira DM, et al. Virus Genes. 2003 Jan;26(1):83-7. doi: 10.1023/a:1022386323921. Virus Genes. 2003. PMID: 12680696
Cited by
- Bovine papillomavirus in Brazil: detection of coinfection of unusual types by a PCR-RFLP method.
Carvalho RF, Sakata ST, Giovanni DN, Mori E, Brandão PE, Richtzenhain LJ, Pozzi CR, Arcaro JR, Miranda MS, Mazzuchelli-de-Souza J, Melo TC, Comenale G, Assaf SL, Beçak W, Stocco RC. Carvalho RF, et al. Biomed Res Int. 2013;2013:270898. doi: 10.1155/2013/270898. Epub 2013 Jun 24. Biomed Res Int. 2013. PMID: 23865043 Free PMC article. - Detection and typing of human papilloma virus by multiplex PCR with type-specific primers.
Romero-Pastrana F. Romero-Pastrana F. ISRN Microbiol. 2012 Mar 1;2012:186915. doi: 10.5402/2012/186915. Print 2012. ISRN Microbiol. 2012. PMID: 23724318 Free PMC article. - Prevalence of HPV high and low risk types in cervical samples from the Italian general population: a population based study.
Giorgi Rossi P, Bisanzi S, Paganini I, Di Iasi A, Angeloni C, Scalisi A, Macis R, Pini MT, Chini F, Carozzi FM; HPV Prevalence Italian Working Group. Giorgi Rossi P, et al. BMC Infect Dis. 2010 Jul 20;10:214. doi: 10.1186/1471-2334-10-214. BMC Infect Dis. 2010. PMID: 20646310 Free PMC article. - Identification and characterization of two novel human papillomaviruses (HPVs) by overlapping PCR: HPV102 and HPV106.
Chen Z, Schiffman M, Herrero R, Burk RD. Chen Z, et al. J Gen Virol. 2007 Nov;88(Pt 11):2952-2955. doi: 10.1099/vir.0.83178-0. J Gen Virol. 2007. PMID: 17947516 Free PMC article. - Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay.
Stephensen CB, Casebolt DB, Gangopadhyay NN. Stephensen CB, et al. Virus Res. 1999 Apr;60(2):181-9. doi: 10.1016/s0168-1702(99)00017-9. Virus Res. 1999. PMID: 10392726 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials