High expression of the chemokine receptor CCR3 in human blood basophils. Role in activation by eotaxin, MCP-4, and other chemokines - PubMed (original) (raw)

High expression of the chemokine receptor CCR3 in human blood basophils. Role in activation by eotaxin, MCP-4, and other chemokines

M Uguccioni et al. J Clin Invest. 1997.

Abstract

Eosinophil leukocytes express high numbers of the chemokine receptor CCR3 which binds eotaxin, monocyte chemotactic protein (MCP)-4, and some other CC chemokines. In this paper we show that CCR3 is also highly expressed on human blood basophils, as indicated by Northern blotting and flow cytometry, and mediates mainly chemotaxis. Eotaxin and MCP-4 elicited basophil migration in vitro with similar efficacy as regulated upon activation normal T cells expressed and secreted (RANTES) and MCP-3. They also induced the release of histamine and leukotrienes in IL-3-primed basophils, but their efficacy was lower than that of MCP-1 and MCP-3, which were the most potent stimuli of exocytosis. Pretreatment of the basophils with a CCR3-blocking antibody abrogated the migration induced by eotaxin, RANTES, and by low to optimal concentrations of MCP-4, but decreased only minimally the response to MCP-3. The CCR3-blocking antibody also affected exocytosis: it abrogated histamine and leukotriene release induced by eotaxin, and partially inhibited the response to RANTES and MCP-4. In contrast, the antibody did not affect the responses induced by MCP-1, MCP-3, and macrophage inflammatory protein-1alpha, which may depend on CCR1 and CCR2, two additional receptors detected by Northern blotting with basophil RNA. This study demonstrates that CCR3 is the major receptor for eotaxin, RANTES, and MCP-4 in human basophils, and suggests that basophils and eosinophils, which are the characteristic effector cells of allergic inflammation, depend largely on CCR3 for migration towards different chemokines into inflamed tissues.

PubMed Disclaimer

Similar articles

• C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other C-C chemokines (monocyte chemoattractant protein-3 and RANTES).
  Ying S, Robinson DS, Meng Q, Barata LT, McEuen AR, Buckley MG, Walls AF, Askenase PW, Kay AB. Ying S, et al. J Immunol. 1999 Oct 1;163(7):3976-84. J Immunol. 1999. PMID: 10491000
• Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody.
  Heath H, Qin S, Rao P, Wu L, LaRosa G, Kassam N, Ponath PD, Mackay CR. Heath H, et al. J Clin Invest. 1997 Jan 15;99(2):178-84. doi: 10.1172/JCI119145. J Clin Invest. 1997. PMID: 9005985 Free PMC article.
• Differential regulation of eosinophil chemokine signaling via CCR3 and non-CCR3 pathways.
  Sabroe I, Hartnell A, Jopling LA, Bel S, Ponath PD, Pease JE, Collins PD, Williams TJ. Sabroe I, et al. J Immunol. 1999 Mar 1;162(5):2946-55. J Immunol. 1999. PMID: 10072545
• [Chemokine (RANTES, eotaxin, etc.)].
  Saito N, Chihara J. Saito N, et al. Nihon Rinsho. 2001 Oct;59(10):1900-5. Nihon Rinsho. 2001. PMID: 11676129 Review. Japanese.
• Role of human FcepsilonRI+ cells in HIV-1 infection.
  Marone G, Florio G, Petraroli A, Triggiani M, de Paulis A. Marone G, et al. Immunol Rev. 2001 Feb;179:128-38. doi: 10.1034/j.1600-065x.2001.790113.x. Immunol Rev. 2001. PMID: 11292016 Review.

Cited by

• Emerging role of human basophil biology in health and disease.
  Cromheecke JL, Nguyen KT, Huston DP. Cromheecke JL, et al. Curr Allergy Asthma Rep. 2014 Jan;14(1):408. doi: 10.1007/s11882-013-0408-2. Curr Allergy Asthma Rep. 2014. PMID: 24346805 Free PMC article. Review.
• Antileukotriene reverts the early effects of inflammatory response of distal parenchyma in experimental chronic allergic inflammation.
  Gobbato NB, de Souza FC, Fumagalli SB, Lopes FD, Prado CM, Martins MA, Tibério Ide F, Leick EA. Gobbato NB, et al. Biomed Res Int. 2013;2013:523761. doi: 10.1155/2013/523761. Epub 2013 Sep 15. Biomed Res Int. 2013. PMID: 24151607 Free PMC article.
• Small molecule receptor agonists and antagonists of CCR3 provide insight into mechanisms of chemokine receptor activation.
  Wise EL, Duchesnes C, da Fonseca PC, Allen RA, Williams TJ, Pease JE. Wise EL, et al. J Biol Chem. 2007 Sep 21;282(38):27935-43. doi: 10.1074/jbc.M703255200. Epub 2007 Jul 16. J Biol Chem. 2007. PMID: 17635911 Free PMC article.
• Bioinformatic analysis of eosinophil activity and its implications for model and target species.
  Jenvey CJ, Alenizi D, Almasi F, Cairns C, Holmes A, Sloan S, Stear MJ. Jenvey CJ, et al. Parasitology. 2020 Apr;147(4):393-400. doi: 10.1017/S0031182019001768. Epub 2019 Dec 16. Parasitology. 2020. PMID: 31839015 Free PMC article. Review.
• The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis.
  Pum A, Ennemoser M, Gerlza T, Kungl AJ. Pum A, et al. Int J Mol Sci. 2022 Jun 10;23(12):6519. doi: 10.3390/ijms23126519. Int J Mol Sci. 2022. PMID: 35742962 Free PMC article.

References

1. 1. Annu Rev Immunol. 1997;15:675-705 - PubMed
2. 1. FEBS Lett. 1997 Jan 6;400(3):329-32 - PubMed
3. 1. Biochemistry. 1991 Mar 26;30(12):3128-35 - PubMed
4. 1. J Biol Chem. 1991 Jun 15;266(17):11245-51 - PubMed
5. 1. J Immunol. 1991 Dec 1;147(11):3855-61 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Society for Clinical Investigation
  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Guide to Pharmacology

• Miscellaneous

  • NCI CPTAC Assay Portal