Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H - PubMed (original) (raw)
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H
L V Wray Jr et al. J Bacteriol. 1997 Sep.
Abstract
Expression of urease, which is encoded by the ureABC operon, is regulated in response to nitrogen availability in Bacillus subtilis. Three ureABC promoters were identified in primer extension experiments and by examination of beta-galactosidase expression from ure-lacZ fusions. P1, a low-level constitutive promoter, lies immediately upstream of ureA. The P2 promoter is transcribed by the E sigmaH form of RNA polymerase and initiates transcription 270 bp upstream of the ureA start codon. The transcriptional start site for the sigmaA-dependent P3 promoter is located 839 bp upstream of the ureA start codon. To identify transcription factors that control ureABC expression, regulation of the P2 and P3 promoters was examined in wild-type and mutant strains. During rapid growth in minimal medium containing glucose and amino acids, CodY represses expression of the P2 and P3 promoters 30- and 60-fold, respectively. TnrA activates expression of the P3 promoter 10-fold in nitrogen-limited cells, while GlnR represses transcription from the P3 promoter 55-fold during growth on excess nitrogen. Expression of the ureABC operon increases 10-fold at the end of exponential growth in nutrient sporulation medium. This elevation in expression results from the relief of CodY-mediated repression during exponential growth and increased sigmaH-dependent transcription during stationary phase.
Similar articles
- Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
Brandenburg JL, Wray LV Jr, Beier L, Jarmer H, Saxild HH, Fisher SH. Brandenburg JL, et al. J Bacteriol. 2002 Nov;184(21):6060-4. doi: 10.1128/JB.184.21.6060-6064.2002. J Bacteriol. 2002. PMID: 12374841 Free PMC article. - Elaborate transcription regulation of the Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA.
Tojo S, Satomura T, Morisaki K, Deutscher J, Hirooka K, Fujita Y. Tojo S, et al. Mol Microbiol. 2005 Jun;56(6):1560-73. doi: 10.1111/j.1365-2958.2005.04635.x. Mol Microbiol. 2005. PMID: 15916606 - Proteus mirabilis urease: operon fusion and linker insertion analysis of ure gene organization, regulation, and function.
Island MD, Mobley HL. Island MD, et al. J Bacteriol. 1995 Oct;177(19):5653-60. doi: 10.1128/jb.177.19.5653-5660.1995. J Bacteriol. 1995. PMID: 7559355 Free PMC article. - Regulation of nitrogen metabolism in Bacillus subtilis: vive la différence!
Fisher SH. Fisher SH. Mol Microbiol. 1999 Apr;32(2):223-32. doi: 10.1046/j.1365-2958.1999.01333.x. Mol Microbiol. 1999. PMID: 10231480 Review. - Spx, a versatile regulator of the Bacillus subtilis stress response.
Schäfer H, Turgay K. Schäfer H, et al. Curr Genet. 2019 Aug;65(4):871-876. doi: 10.1007/s00294-019-00950-6. Epub 2019 Mar 4. Curr Genet. 2019. PMID: 30830258 Review.
Cited by
- Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO3 Precipitation.
Carter MS, Tuttle MJ, Mancini JA, Martineau R, Hung CS, Gupta MK. Carter MS, et al. Appl Environ Microbiol. 2023 Aug 30;89(8):e0179422. doi: 10.1128/aem.01794-22. Epub 2023 Jul 13. Appl Environ Microbiol. 2023. PMID: 37439668 Free PMC article. Review. - Prophylactic immunization to Helicobacter pylori infection using spore vectored vaccines.
Katsande PM, Nguyen VD, Nguyen TLP, Nguyen TKC, Mills G, Bailey DMD, Christie G, Hong HA, Cutting SM. Katsande PM, et al. Helicobacter. 2023 Aug;28(4):e12997. doi: 10.1111/hel.12997. Epub 2023 Jun 14. Helicobacter. 2023. PMID: 37314018 Free PMC article. - Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective.
He H, Li Y, Zhang L, Ding Z, Shi G. He H, et al. J Adv Res. 2023 Jul;49:1-14. doi: 10.1016/j.jare.2022.09.003. Epub 2022 Sep 12. J Adv Res. 2023. PMID: 36103961 Free PMC article. Review. - Is hyaluronic acid production transcriptionally regulated? A transcriptional repressor gene deletion study in Streptococcus zooepidemicus.
Gao W, Zhang X, Zhang G, Zuo M, Cao W, Xie Z, Liu H. Gao W, et al. Appl Microbiol Biotechnol. 2021 Nov;105(21-22):8495-8504. doi: 10.1007/s00253-021-11481-x. Epub 2021 Oct 18. Appl Microbiol Biotechnol. 2021. PMID: 34661707 - GlnR Negatively Regulates Glutamate-Dependent Acid Resistance in Lactobacillus brevis.
Gong L, Ren C, Xu Y. Gong L, et al. Appl Environ Microbiol. 2020 Mar 18;86(7):e02615-19. doi: 10.1128/AEM.02615-19. Print 2020 Mar 18. Appl Environ Microbiol. 2020. PMID: 31953336 Free PMC article.
References
- J Bacteriol. 1995 Jun;177(12):3546-55 - PubMed
- Gene. 1986;43(1-2):85-94 - PubMed
- J Mol Biol. 1995 Jun 23;249(5):843-56 - PubMed
- Mol Microbiol. 1995 Apr;16(1):145-55 - PubMed
- Microbiol Rev. 1995 Sep;59(3):451-80 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases