SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs - PubMed (original) (raw)

. 1997 Aug 22;195(2):151-9.

doi: 10.1016/s0378-1119(97)00121-2.

B C Lee, A Zaballos, A Peled, I Barthelemy, Y Shav-Tal, I Prieto, P Carmi, Y Gothelf, G González de Buitrago, M Aracil, G Márquez, J L Barbero, D Zipori

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SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs

L Carramolino et al. Gene. 1997.

Erratum in

Abstract

We report the molecular cloning of a novel gene family. The first member of this family was cloned from a mouse lambda gt11 cDNA library using the B92 monoclonal antibody (mAb) raised against stromal cell extracts. This was followed by RACE-PCR using mRNA from the stromal cell line. A 4 kb cDNA was obtained encoding a unique protein sequence of 1258 aa, that we designate stromal antigen (SA)-1. The human SA-1 gene was cloned by homology from a thymus cDNA library and the sequence of the predicted protein was found to be highly homologous to the murine SA-1 (>98.9%). Another cDNA was cloned and the deduced protein (SA-2) was 71% homologous to SA-1. Northern blot and PCR analysis indicated that on the mRNA level the SA-1 gene is expressed in all tissues analyzed and probably encodes a single transcript. The identification of SA-1 protein in tissues and cells required combined immunoprecipitation and Western blotting using a polyclonal antiserum raised against a predicted peptide of SA-1 and the B92 mAb. Using this assay we identified a protein of about 120 kDa in hemopoietic organs. Subcellular fractionation indicated that SA-1 is a nuclear protein. Thus, despite the ubiquitous expression on the mRNA level, the protein was predominantly detected in hemopoietic organs and may therefore be controlled on a post-transcriptional level. The SA-1 gene described in this study is highly conserved between mouse and man. This implies a crucial function for this protein.

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