Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome - PubMed (original) (raw)
Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome
X W Yang et al. Nat Biotechnol. 1997 Sep.
Abstract
Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artificial chromosomes directly in the recombination-deficient E. coli host strain by homologous recombination for in vivo studies. The IRES-LacZ marker gene was introduced into a 131 kb BAC containing the murine zinc finger gene, RU49. No rearrangements or deletions were detected in the modified BACs. Furthermore, transgenic mice were generated by pronuclear injection of the modified BAC, and germline transmission of the intact BAC has been obtained. Proper expression of the lacZ transgene in the brain has been observed, which could not be obtained with conventional transgenic constructs.
Comment in
- Dysfunctional genomics: BACs to the rescue.
Simon MI. Simon MI. Nat Biotechnol. 1997 Sep;15(9):839. doi: 10.1038/nbt0997-839. Nat Biotechnol. 1997. PMID: 9306392 No abstract available.
Similar articles
- BAC trimming: minimizing clone overlaps.
Hill F, Benes V, Thomasova D, Stewart AF, Kafatos FC, Ansorge W. Hill F, et al. Genomics. 2000 Feb 15;64(1):111-3. doi: 10.1006/geno.1999.6106. Genomics. 2000. PMID: 10708525 - Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells.
Heaney JD, Rettew AN, Bronson SK. Heaney JD, et al. Genomics. 2004 Jun;83(6):1072-82. doi: 10.1016/j.ygeno.2003.12.015. Genomics. 2004. PMID: 15177560 - Engineering large fragment insertions into the chromosome of Escherichia coli.
Rong R, Slupska MM, Chiang JH, Miller JH. Rong R, et al. Gene. 2004 Jul 7;336(1):73-80. doi: 10.1016/j.gene.2004.02.054. Gene. 2004. PMID: 15225877 - BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.
Sparwasser T, Eberl G. Sparwasser T, et al. Immunology. 2007 Jul;121(3):308-13. doi: 10.1111/j.1365-2567.2007.02605.x. Epub 2007 Apr 16. Immunology. 2007. PMID: 17437533 Free PMC article. Review. - An overview on the generation of BAC transgenic mice for neuroscience research.
Yang XW, Gong S. Yang XW, et al. Curr Protoc Neurosci. 2005 May;Chapter 5:Unit 5.20. doi: 10.1002/0471142301.ns0520s31. Curr Protoc Neurosci. 2005. PMID: 18428622 Review.
Cited by
- NPAS4 supports cocaine-conditioned cues in rodents by controlling the cell type-specific activation balance in the nucleus accumbens.
Hughes BW, Huebschman JL, Tsvetkov E, Siemsen BM, Snyder KK, Akiki RM, Wood DJ, Penrod RD, Scofield MD, Berto S, Taniguchi M, Cowan CW. Hughes BW, et al. Nat Commun. 2024 Aug 8;15(1):5971. doi: 10.1038/s41467-024-50099-1. Nat Commun. 2024. PMID: 39117647 Free PMC article. - Updates on mouse models of Alzheimer's disease.
Zhong MZ, Peng T, Duarte ML, Wang M, Cai D. Zhong MZ, et al. Mol Neurodegener. 2024 Mar 11;19(1):23. doi: 10.1186/s13024-024-00712-0. Mol Neurodegener. 2024. PMID: 38462606 Free PMC article. Review. - Precise genome-editing in human diseases: mechanisms, strategies and applications.
Zheng Y, Li Y, Zhou K, Li T, VanDusen NJ, Hua Y. Zheng Y, et al. Signal Transduct Target Ther. 2024 Feb 26;9(1):47. doi: 10.1038/s41392-024-01750-2. Signal Transduct Target Ther. 2024. PMID: 38409199 Free PMC article. Review. - The widely used Ucp1-Cre Evdr transgene elicits complex developmental and metabolic phenotypes.
Halurkar MS, Inoue O, Mukherjee R, Paese CLB, Duszynski M, Brugmann SA, Lim HW, Sanchez-Gurmaches J. Halurkar MS, et al. bioRxiv [Preprint]. 2023 Oct 20:2023.10.20.563165. doi: 10.1101/2023.10.20.563165. bioRxiv. 2023. PMID: 37904917 Free PMC article. Preprint. - Strategies to identify and edit improvements in synthetic genome segments episomally.
Rudolph A, Nyerges A, Chiappino-Pepe A, Landon M, Baas-Thomas M, Church G. Rudolph A, et al. Nucleic Acids Res. 2023 Oct 13;51(18):10094-10106. doi: 10.1093/nar/gkad692. Nucleic Acids Res. 2023. PMID: 37615546 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources