An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal - PubMed (original) (raw)
An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal
N Hui et al. Mol Biol Cell. 1997 Sep.
Free PMC article
Abstract
The early Golgi t-SNARE (target-membrane-associated soluble-N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 5 is thought to specify the docking site for both COPI and COPII coated vesicles originating from the endoplasmic reticulum (ER) and COPI vesicles on the retrograde pathway. We now show that there are two forms of syntaxin 5 that appear to be generated from the same mRNA by alternative initiation of translation. The short form (35 kDa) corresponds to the published sequence. The long form (42 kDa) has an N-terminal cytoplasmic extension containing a predicted type II ER retrieval signal. When grafted onto a reporter molecule, this signal localized the construct to the ER. Biochemical fractionation and immunofluorescence microscopy showed that there was less of the long form in the Golgi apparatus and more in peripheral punctate structures, some of which colocalized with markers of the intermediate compartment. The predicted absence of the long form in budding yeast points to a function unique to higher organisms.
Similar articles
- Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs.
Hay JC, Klumperman J, Oorschot V, Steegmaier M, Kuo CS, Scheller RH. Hay JC, et al. J Cell Biol. 1998 Jun 29;141(7):1489-502. doi: 10.1083/jcb.141.7.1489. J Cell Biol. 1998. PMID: 9647643 Free PMC article. - Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking.
Hatsuzawa K, Hirose H, Tani K, Yamamoto A, Scheller RH, Tagaya M. Hatsuzawa K, et al. J Biol Chem. 2000 May 5;275(18):13713-20. doi: 10.1074/jbc.275.18.13713. J Biol Chem. 2000. PMID: 10788491 - Members of a mammalian SNARE complex interact in the endoplasmic reticulum in vivo and are found in COPI vesicles.
Verrier SE, Willmann M, Wenzel D, Winter U, von Mollard GF, Söling HD. Verrier SE, et al. Eur J Cell Biol. 2008 Nov;87(11):863-78. doi: 10.1016/j.ejcb.2008.07.003. Epub 2008 Oct 1. Eur J Cell Biol. 2008. PMID: 18834646 - Stx5-Mediated ER-Golgi Transport in Mammals and Yeast.
Linders PT, Horst CV, Beest MT, van den Bogaart G. Linders PT, et al. Cells. 2019 Jul 26;8(8):780. doi: 10.3390/cells8080780. Cells. 2019. PMID: 31357511 Free PMC article. Review. - Getting into the Golgi.
Bannykh SI, Nishimura N, Balch WE. Bannykh SI, et al. Trends Cell Biol. 1998 Jan;8(1):21-5. doi: 10.1016/s0962-8924(97)01184-7. Trends Cell Biol. 1998. PMID: 9695803 Review.
Cited by
- STX5 Inhibits Hepatocellular Carcinoma Adhesion and Promotes Metastasis by Regulating the PI3K/mTOR Pathway.
Zhang B, Zhao Z, Wang Y, Guo T, He M, Guan G, Peng P, Cai J, Zhang B, Liu X, Song Q. Zhang B, et al. J Clin Transl Hepatol. 2023 Jun 28;11(3):572-583. doi: 10.14218/JCTH.2022.00276. Epub 2023 Jan 9. J Clin Transl Hepatol. 2023. PMID: 36969886 Free PMC article. - Revisiting Leishmania GP63 host cell targets reveals a limited spectrum of substrates.
Guay-Vincent MM, Matte C, Berthiaume AM, Olivier M, Jaramillo M, Descoteaux A. Guay-Vincent MM, et al. PLoS Pathog. 2022 Oct 3;18(10):e1010640. doi: 10.1371/journal.ppat.1010640. eCollection 2022 Oct. PLoS Pathog. 2022. PMID: 36191034 Free PMC article. - Syntaxin 5 determines Weibel-Palade body size and von Willebrand factor secretion by controlling Golgi architecture.
Kat M, Karampini E, Hoogendijk AJ, Bürgisser PE, Mulder AA, Van Alphen FPJ, Olins J, Geerts D, Van den Biggelaar M, Margadant C, Voorberg J, Bierings R. Kat M, et al. Haematologica. 2022 Aug 1;107(8):1827-1839. doi: 10.3324/haematol.2021.280121. Haematologica. 2022. PMID: 35081689 Free PMC article. - Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5.
Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Õunap K, Ter Beest M, Lefeber D, van den Bogaart G. Linders PTA, et al. Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y. Nat Commun. 2021. PMID: 34711829 Free PMC article. - Ipomoeassin-F disrupts multiple aspects of secretory protein biogenesis.
Roboti P, O'Keefe S, Duah KB, Shi WQ, High S. Roboti P, et al. Sci Rep. 2021 Jun 2;11(1):11562. doi: 10.1038/s41598-021-91107-4. Sci Rep. 2021. PMID: 34079010 Free PMC article.
References
- Mol Cell Biol. 1991 Jun;11(6):2980-93 - PubMed
- Cell. 1996 Apr 5;85(1):83-94 - PubMed
- EMBO J. 1991 Dec;10(12):3567-75 - PubMed
- Yeast. 1991 Aug-Sep;7(6):643-50 - PubMed
- Cell. 1992 Jan 24;68(2):181-2 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases