Functional analysis of the nuclear LIM domain interactor NLI - PubMed (original) (raw)

Functional analysis of the nuclear LIM domain interactor NLI

L W Jurata et al. Mol Cell Biol. 1997 Oct.

Abstract

LIM homeodomain and LIM-only (LMO) transcription factors contain two tandemly arranged Zn2+-binding LIM domains capable of mediating protein-protein interactions. These factors have restricted patterns of expression, are found in invertebrates as well as vertebrates, and are required for cell type specification in a variety of developing tissues. A recently identified, widely expressed protein, NLI, binds with high affinity to the LIM domains of LIM homeodomain and LMO proteins in vitro and in vivo. In this study, a 38-amino-acid fragment of NLI was found to be sufficient for the association of NLI with nuclear LIM domains. In addition, NLI was shown to form high affinity homodimers through the amino-terminal 200 amino acids, but dimerization of NLI was not required for association with the LIM homeodomain protein Lmxl. Chemical cross-linking analysis revealed higher-order complexes containing multiple NLI molecules bound to Lmx1, indicating that dimerization of NLI does not interfere with LIM domain interactions. Additionally, NLI formed complexes with Lmx1 on the rat insulin I promoter and inhibited the LIM domain-dependent synergistic transcriptional activation by Lmx1 and the basic helix-loop-helix protein E47 from the rat insulin I minienhancer. These studies indicate that NLI contains at least two functionally independent domains and may serve as a negative regulator of synergistic transcriptional responses which require direct interaction via LIM domains. Thus, NLI may regulate the transcriptional activity of LIM homeodomain proteins by determining specific partner interactions.

PubMed Disclaimer

Cited by

SEUSS and SEUSS-LIKE transcriptional adaptors regulate floral and embryonic development in Arabidopsis.
Bao F, Azhakanandam S, Franks RG. Bao F, et al. Plant Physiol. 2010 Feb;152(2):821-36. doi: 10.1104/pp.109.146183. Epub 2009 Dec 9. Plant Physiol. 2010. PMID: 20007451 Free PMC article.
Lmo4 and Clim1 progressively delineate cortical projection neuron subtypes during development.
Azim E, Shnider SJ, Cederquist GY, Sohur US, Macklis JD. Azim E, et al. Cereb Cortex. 2009 Jul;19 Suppl 1(Suppl 1):i62-9. doi: 10.1093/cercor/bhp030. Epub 2009 Apr 14. Cereb Cortex. 2009. PMID: 19366868 Free PMC article.
Cell-specific repressor or enhancer activities of Deaf-1 at a serotonin 1A receptor gene polymorphism.
Czesak M, Lemonde S, Peterson EA, Rogaeva A, Albert PR. Czesak M, et al. J Neurosci. 2006 Feb 8;26(6):1864-71. doi: 10.1523/JNEUROSCI.2643-05.2006. J Neurosci. 2006. PMID: 16467535 Free PMC article.
Requirement for Lmo4 in the vestibular morphogenesis of mouse inner ear.
Deng M, Pan L, Xie X, Gan L. Deng M, et al. Dev Biol. 2010 Feb 1;338(1):38-49. doi: 10.1016/j.ydbio.2009.11.003. Epub 2009 Nov 10. Dev Biol. 2010. PMID: 19913004 Free PMC article.
A positive role for NLI/Ldb1 in long-range beta-globin locus control region function.
Song SH, Hou C, Dean A. Song SH, et al. Mol Cell. 2007 Dec 14;28(5):810-22. doi: 10.1016/j.molcel.2007.09.025. Mol Cell. 2007. PMID: 18082606 Free PMC article.

References

1. Mol Endocrinol. 1991 Feb;5(2):292-9 - PubMed
1. Nature. 1990 Apr 26;344(6269):876-9 - PubMed
1. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):38-42 - PubMed
1. Mol Cell Biol. 1992 Mar;12(3):1031-42 - PubMed
1. Mol Cell Biol. 1992 Apr;12(4):1777-88 - PubMed

Functional analysis of the nuclear LIM domain interactor NLI - PubMed (original) (raw)