Dihydrofolate reductase protein inhibits its own translation by binding to dihydrofolate reductase mRNA sequences within the coding region - PubMed (original) (raw)
. 1997 Oct 7;36(40):12317-22.
doi: 10.1021/bi971026e.
Affiliations
- PMID: 9315871
- DOI: 10.1021/bi971026e
Dihydrofolate reductase protein inhibits its own translation by binding to dihydrofolate reductase mRNA sequences within the coding region
E A Ercikan-Abali et al. Biochemistry. 1997.
Abstract
Previous studies suggest that dihydrofolate reductase (DHFR) regulates its own translation. Moreover, intracellular levels of DHFR protein increase following exposure to the antifolate methotrexate (MTX), suggesting that MTX may release the translational inhibition mediated by DHFR [Chu et al. (1993) Biochemistry 32,4756-4760; Ercikan et al. (1993) Adv. Exp. Med. Biol. 338, 537-540]. To further investigate the role of DHFR in translational autoregulation, we have considered the possibility that DHFR directly contacts its cognate mRNA. Binding studies using a series of truncated DHFR mRNAs as probes localized the DHFR/RNA interaction to a 100-base-pair region containing two putative stem-loop structures; initial studies indicated that both of these loop structures are involved in protein binding. Moreover, the binding of MTX to DHFR prevents interaction of the protein with its cognate mRNA, thereby relieving translational autoregulation.
Similar articles
- Molecular mechanisms of resistance to antifolates, a review.
Banerjee D, Ercikan-Abali E, Waltham M, Schnieders B, Hochhauser D, Li WW, Fan J, Gorlick R, Goker E, Bertino JR. Banerjee D, et al. Acta Biochim Pol. 1995;42(4):457-64. Acta Biochim Pol. 1995. PMID: 8852336 Review. - Identification of a cis-acting element of human dihydrofolate reductase mRNA.
Tai N, Schmitz JC, Chen TM, O'Neill MB, Chu E. Tai N, et al. Biochem Biophys Res Commun. 2008 May 9;369(3):795-800. doi: 10.1016/j.bbrc.2007.09.044. Epub 2007 Sep 21. Biochem Biophys Res Commun. 2008. PMID: 18045573 - Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro.
Chu E, Takimoto CH, Voeller D, Grem JL, Allegra CJ. Chu E, et al. Biochemistry. 1993 May 11;32(18):4756-60. doi: 10.1021/bi00069a009. Biochemistry. 1993. PMID: 8490020 - Characterization and comparative studies of zebrafish and human recombinant dihydrofolate reductases--inhibition by folic acid and polyphenols.
Kao TT, Wang KC, Chang WN, Lin CY, Chen BH, Wu HL, Shi GY, Tsai JN, Fu TF. Kao TT, et al. Drug Metab Dispos. 2008 Mar;36(3):508-16. doi: 10.1124/dmd.107.019299. Epub 2007 Dec 3. Drug Metab Dispos. 2008. PMID: 18056255 - Drug-resistant dihydrofolate reductases: generation, expression and therapeutic application.
McIvor RS. McIvor RS. Bone Marrow Transplant. 1996 Dec;18 Suppl 3:S50-4. Bone Marrow Transplant. 1996. PMID: 8971409 Review.
Cited by
- Unveiling the Therapeutic Potential of Folate-Dependent One-Carbon Metabolism in Cancer and Neurodegeneration.
Sobral AF, Cunha A, Silva V, Gil-Martins E, Silva R, Barbosa DJ. Sobral AF, et al. Int J Mol Sci. 2024 Aug 28;25(17):9339. doi: 10.3390/ijms25179339. Int J Mol Sci. 2024. PMID: 39273288 Free PMC article. Review. - Decrypting the molecular basis of cellular drug phenotypes by dose-resolved expression proteomics.
Eckert S, Berner N, Kramer K, Schneider A, Müller J, Lechner S, Brajkovic S, Sakhteman A, Graetz C, Fackler J, Dudek M, Pfaffl MW, Knolle P, Wilhelm S, Kuster B. Eckert S, et al. Nat Biotechnol. 2024 May 7. doi: 10.1038/s41587-024-02218-y. Online ahead of print. Nat Biotechnol. 2024. PMID: 38714896 - Regulatory mechanisms of one-carbon metabolism enzymes.
Petrova B, Maynard AG, Wang P, Kanarek N. Petrova B, et al. J Biol Chem. 2023 Dec;299(12):105457. doi: 10.1016/j.jbc.2023.105457. Epub 2023 Nov 9. J Biol Chem. 2023. PMID: 37949226 Free PMC article. Review. - Methotrexate-based PROTACs as DHFR-specific chemical probes.
Rana S, Dranchak P, Dahlin JL, Lamy L, Li W, Oliphant E, Shrimp JH, Rajacharya GH, Tharakan R, Holland DO, Whitten AS, Wilson KM, Singh PK, Durum SK, Tao D, Rai G, Inglese J. Rana S, et al. Cell Chem Biol. 2024 Feb 15;31(2):221-233.e14. doi: 10.1016/j.chembiol.2023.09.014. Epub 2023 Oct 23. Cell Chem Biol. 2024. PMID: 37875111 - Cycloguanil and Analogues Potently Target DHFR in Cancer Cells to Elicit Anti-Cancer Activity.
Brown JI, Wang P, Wong AYL, Petrova B, Persaud R, Soukhtehzari S, Lopez McDonald M, Hanke D, Christensen J, Iliev P, Wang W, Everton DK, Williams KC, Frank DA, Kanarek N, Page BDG. Brown JI, et al. Metabolites. 2023 Jan 19;13(2):151. doi: 10.3390/metabo13020151. Metabolites. 2023. PMID: 36837770 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources