Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages - PubMed (original) (raw)

Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages

P A Denoel et al. Infect Immun. 1997 Oct.

Abstract

A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.

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