Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast - PubMed (original) (raw)
Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast
S B Hua et al. Plasmid. 1997.
Abstract
With efficient homologous recombination in Saccharomyces cerevisiae, a rapid in vivo cloning technique has been available. Here we demonstrated that 30 bp of a homologous sequence at each end of a DNA fragment is sufficient to integrate the fragment into a linearized plasmid in yeast. To obtain a high yield of recombination transformants, however, more than 60 bp are desirable. Interestingly, we observed that 20 bp of homology at one end of a DNA fragment is sufficient for efficient recombination provided that the other end contains 80 bp of homologous sequence. Some applications, including high-throughput transferring of EST inserts to the yeast expression systems for the Human Genome Project, are discussed.
Similar articles
- In vivo cloning by homologous recombination in yeast using a two-plasmid-based system.
Degryse E, Dumas B, Dietrich M, Laruelle L, Achstetter T. Degryse E, et al. Yeast. 1995 Jun 15;11(7):629-40. doi: 10.1002/yea.320110704. Yeast. 1995. PMID: 7483836 - Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.
Ito K, Sugawara T, Shiroishi M, Tokuda N, Kurokawa A, Misaka T, Makyio H, Yurugi-Kobayashi T, Shimamura T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T. Ito K, et al. Biochem Biophys Res Commun. 2008 Jul 11;371(4):841-5. doi: 10.1016/j.bbrc.2008.04.182. Epub 2008 May 12. Biochem Biophys Res Commun. 2008. PMID: 18474222 - High-throughput cloning of human liver complete open reading frames using homologous recombination in Escherichia coli.
Zhu D, Zhong X, Tan R, Chen L, Huang G, Li J, Sun X, Xu L, Chen J, Ou Y, Zhang T, Yuan D, Zhang Z, Shu W, Ma L. Zhu D, et al. Anal Biochem. 2010 Feb 15;397(2):162-7. doi: 10.1016/j.ab.2009.10.018. Epub 2009 Oct 14. Anal Biochem. 2010. PMID: 19835833 - TAR cloning: insights into gene function, long-range haplotypes and genome structure and evolution.
Kouprina N, Larionov V. Kouprina N, et al. Nat Rev Genet. 2006 Oct;7(10):805-12. doi: 10.1038/nrg1943. Nat Rev Genet. 2006. PMID: 16983376 Review. - Finding a match: how do homologous sequences get together for recombination?
Barzel A, Kupiec M. Barzel A, et al. Nat Rev Genet. 2008 Jan;9(1):27-37. doi: 10.1038/nrg2224. Nat Rev Genet. 2008. PMID: 18040271 Review.
Cited by
- Eukaryotic resectosomes: A single-molecule perspective.
Myler LR, Finkelstein IJ. Myler LR, et al. Prog Biophys Mol Biol. 2017 Aug;127:119-129. doi: 10.1016/j.pbiomolbio.2016.08.001. Epub 2016 Aug 3. Prog Biophys Mol Biol. 2017. PMID: 27498169 Free PMC article. Review. - Identification of a bHLH-type G-box binding factor and its regulation activity with G-box and Box I elements of the PsCHS1 promoter.
Qian W, Tan G, Liu H, He S, Gao Y, An C. Qian W, et al. Plant Cell Rep. 2007 Jan;26(1):85-93. doi: 10.1007/s00299-006-0202-x. Epub 2006 Aug 22. Plant Cell Rep. 2007. PMID: 16924502 - Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein.
Rao L, Hülsemann M, Gennerich A. Rao L, et al. Methods Mol Biol. 2018;1665:53-89. doi: 10.1007/978-1-4939-7271-5_4. Methods Mol Biol. 2018. PMID: 28940064 Free PMC article. - Minimizing the damage: repair pathways keep mitochondrial DNA intact.
Kazak L, Reyes A, Holt IJ. Kazak L, et al. Nat Rev Mol Cell Biol. 2012 Oct;13(10):659-71. doi: 10.1038/nrm3439. Epub 2012 Sep 20. Nat Rev Mol Cell Biol. 2012. PMID: 22992591 Review. - Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi.
Dahlmann TA, Terfehr D, Becker K, Teichert I. Dahlmann TA, et al. Curr Genet. 2021 Apr;67(2):317-330. doi: 10.1007/s00294-020-01143-2. Epub 2020 Dec 24. Curr Genet. 2021. PMID: 33367953 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials