Cell death suggestive of apoptosis after spinal cord ischemia in rabbits - PubMed (original) (raw)
Cell death suggestive of apoptosis after spinal cord ischemia in rabbits
M E Mackey et al. Stroke. 1997 Oct.
Abstract
Background and purpose: After spinal cord ischemia, some neurons remain viable after an ischemic insult but may be at risk of dying during reperfusion. We searched for morphological and biochemical features of apoptosis, which is a mechanism of delayed neuronal death, in a rabbit model of spinal cord ischemia.
Methods: The infrarenal aorta of White New Zealand rabbits (n = 24) was occluded for 40 minutes using a loop tourniquet. Rabbits were killed after 12, 24, or 48 hours (n = 8 per group). The loop was placed but never tightened in sham-operated rabbits (n = 6). The lumbar segment of the spinal cord (L5 to L7) was used for morphological studies, including hematoxylin and eosin staining and a modified terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) staining method. Electron microscopy was used to examine ultrastructural morphology. In addition, lumbar tissue was used for biochemical investigation of DNA laddering by agarose gel electrophoresis.
Results: After ischemia, the affected areas contained neurons with positive TUNEL staining. Positive neurons were located in laminae III to IX, although most were concentrated in the intermediate and ventral areas. Adjacent sections stained with hematoxylin and eosin exhibited ischemic cell changes (red and ghost neurons), while apoptotic bodies were also apparent. In addition, electron microscopy of ischemic tissue samples exhibited ultrastructural characteristics of apoptosis, including nuclear condensation and relatively normal organelle morphology. Finally, isolated DNA revealed a ladder on agarose gel electrophoresis, indicating DNA fragmentation into approximately 180 multiples of base pairs.
Conclusions: Spinal cord ischemia in rabbits induces morphological and biochemical changes suggestive of apoptosis. These data raise the possibility that apoptosis contributes to neuronal cell death after spinal cord ischemia.
Similar articles
- Riluzole prevents ischemic spinal cord injury caused by aortic crossclamping.
Lang-Lazdunski L, Heurteaux C, Vaillant N, Widmann C, Lazdunski M. Lang-Lazdunski L, et al. J Thorac Cardiovasc Surg. 1999 May;117(5):881-9. doi: 10.1016/S0022-5223(99)70367-3. J Thorac Cardiovasc Surg. 1999. PMID: 10220679 - Neuronal apoptosis and necrosis following spinal cord ischemia in the rat.
Kato H, Kanellopoulos GK, Matsuo S, Wu YJ, Jacquin MF, Hsu CY, Kouchoukos NT, Choi DW. Kato H, et al. Exp Neurol. 1997 Dec;148(2):464-74. doi: 10.1006/exnr.1997.6707. Exp Neurol. 1997. PMID: 9417826 - Apoptosis of motor neurons with induction of caspases in the spinal cord after ischemia.
Hayashi T, Sakurai M, Abe K, Sadahiro M, Tabayashi K, Itoyama Y. Hayashi T, et al. Stroke. 1998 May;29(5):1007-12; discussion 1013. doi: 10.1161/01.str.29.5.1007. Stroke. 1998. PMID: 9596251 - [Ischemic heart disease and apoptosis].
Takemura G, Ohno M, Fujiwara H. Takemura G, et al. Rinsho Byori. 1997 Jul;45(7):606-13. Rinsho Byori. 1997. PMID: 9256005 Review. Japanese. - Apoptotic detection methods--from morphology to gene.
Otsuki Y, Li Z, Shibata MA. Otsuki Y, et al. Prog Histochem Cytochem. 2003;38(3):275-339. doi: 10.1016/s0079-6336(03)80002-5. Prog Histochem Cytochem. 2003. PMID: 12756893 Review.
Cited by
- Beneficial effect of the oxygen free radical scavenger amifostine (WR-2721) on spinal cord ischemia/reperfusion injury in rabbits.
Chronidou F, Apostolakis E, Papapostolou I, Grintzalis K, Georgiou CD, Koletsis EN, Karanikolas M, Papathanasopoulos P, Dougenis D. Chronidou F, et al. J Cardiothorac Surg. 2009 Sep 17;4:50. doi: 10.1186/1749-8090-4-50. J Cardiothorac Surg. 2009. PMID: 19758462 Free PMC article. - Heme Oxygenase-1 Protects Neurons from Ischemic Damage by Upregulating Expression of Cu,Zn-Superoxide Dismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.
Jung HY, Kim DW, Yim HS, Yoo DY, Kim JW, Won MH, Yoon YS, Choi SY, Hwang IK. Jung HY, et al. Neurochem Res. 2016 Apr;41(4):869-79. doi: 10.1007/s11064-015-1764-1. Epub 2015 Nov 11. Neurochem Res. 2016. PMID: 26559686 - Tat-p27 Ameliorates Neuronal Damage Reducing α-Synuclein and Inflammatory Responses in Motor Neurons After Spinal Cord Ischemia.
Kim W, Kwon HJ, Jung HY, Hahn KR, Moon SM, Yoon YS, Hwang IK, Choi SY, Kim DW. Kim W, et al. Neurochem Res. 2021 Dec;46(12):3123-3134. doi: 10.1007/s11064-021-03392-0. Epub 2021 Aug 17. Neurochem Res. 2021. PMID: 34403064 - Cu, Zn-Superoxide Dismutase Increases the Therapeutic Potential of Adipose-derived Mesenchymal Stem Cells by Maintaining Antioxidant Enzyme Levels.
Yoo DY, Kim DW, Chung JY, Jung HY, Kim JW, Yoon YS, Hwang IK, Choi JH, Choi GM, Choi SY, Moon SM. Yoo DY, et al. Neurochem Res. 2016 Dec;41(12):3300-3307. doi: 10.1007/s11064-016-2062-2. Epub 2016 Oct 14. Neurochem Res. 2016. PMID: 27743287 - Fas receptor and neuronal cell death after spinal cord ischemia.
Matsushita K, Wu Y, Qiu J, Lang-Lazdunski L, Hirt L, Waeber C, Hyman BT, Yuan J, Moskowitz MA. Matsushita K, et al. J Neurosci. 2000 Sep 15;20(18):6879-87. doi: 10.1523/JNEUROSCI.20-18-06879.2000. J Neurosci. 2000. PMID: 10995832 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources