Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells - PubMed (original) (raw)
Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells
M Tada et al. EMBO J. 1997.
Abstract
Genomic reprogramming of primordial germ cells (PGCs), which includes genome-wide demethylation, prevents aberrant epigenetic modifications from being transmitted to subsequent generations. This process also ensures that homologous chromosomes first acquire an identical epigenetic status before an appropriate switch in the imprintable loci in the female and male germ lines. Embryonic germ (EG) cells have a similar epigenotype to PGCs from which they are derived. We used EG cells to investigate the mechanism of epigenetic modifications in the germ line by analysing the effects on a somatic nucleus in the EG-thymic lymphocyte hybrid cells. There were striking changes in methylation of the somatic nucleus, resulting in demethylation of several imprinted and non-imprinted genes. These epigenetic modifications were heritable and affected gene expression as judged by re-activation of the silent maternal allele of Peg1/Mest imprinted gene in the somatic nucleus. This remarkable change in the epigenotype of the somatic nucleus is consistent with the observed pluripotency of the EG-somatic hybrid cells as they differentiated into a variety of tissues in chimeric embryos. The epigenetic modifications observed in EG-somatic cell hybrids in vitro are comparable to the reprogramming events that occur during germ cell development.
Similar articles
- Reprogramming a somatic nucleus by trans-modification activity in germ cells.
Surani MA. Surani MA. Semin Cell Dev Biol. 1999 Jun;10(3):273-7. doi: 10.1006/scdb.1998.0285. Semin Cell Dev Biol. 1999. PMID: 10441539 Review. - Epigenotype switching of imprintable loci in embryonic germ cells.
Tada T, Tada M, Hilton K, Barton SC, Sado T, Takagi N, Surani MA. Tada T, et al. Dev Genes Evol. 1998 Feb;207(8):551-61. doi: 10.1007/s004270050146. Dev Genes Evol. 1998. PMID: 9510550 - Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice.
Takehashi M, Tada M, Kanatsu-Shinohara M, Morimoto H, Kazuki Y, Oshimura M, Tada T, Shinohara T. Takehashi M, et al. Biol Reprod. 2012 Jun 14;86(6):178. doi: 10.1095/biolreprod.112.098988. Print 2012 Jun. Biol Reprod. 2012. PMID: 22441799 - A current view of the epigenome in mouse primordial germ cells.
Matsui Y, Mochizuki K. Matsui Y, et al. Mol Reprod Dev. 2014 Feb;81(2):160-70. doi: 10.1002/mrd.22214. Epub 2013 Aug 7. Mol Reprod Dev. 2014. PMID: 23868517 Review. - Erasure of DNA methylation, genomic imprints, and epimutations in a primordial germ-cell model derived from mouse pluripotent stem cells.
Miyoshi N, Stel JM, Shioda K, Qu N, Odajima J, Mitsunaga S, Zhang X, Nagano M, Hochedlinger K, Isselbacher KJ, Shioda T. Miyoshi N, et al. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9545-50. doi: 10.1073/pnas.1610259113. Epub 2016 Aug 2. Proc Natl Acad Sci U S A. 2016. PMID: 27486249 Free PMC article.
Cited by
- DNA synthesis is required for reprogramming mediated by stem cell fusion.
Tsubouchi T, Soza-Ried J, Brown K, Piccolo FM, Cantone I, Landeira D, Bagci H, Hochegger H, Merkenschlager M, Fisher AG. Tsubouchi T, et al. Cell. 2013 Feb 14;152(4):873-83. doi: 10.1016/j.cell.2013.01.012. Cell. 2013. PMID: 23415233 Free PMC article. - Experimental approach to prezygotic chromosome screening using only a single pair of gametes in mice.
Watanabe H, Kohda A, Tateno H. Watanabe H, et al. J Reprod Dev. 2015;61(6):511-8. doi: 10.1262/jrd.2015-023. Epub 2015 Aug 3. J Reprod Dev. 2015. PMID: 26234555 Free PMC article. - Reprogramming and development in nuclear transfer embryos and in interspecific systems.
Narbonne P, Miyamoto K, Gurdon JB. Narbonne P, et al. Curr Opin Genet Dev. 2012 Oct;22(5):450-8. doi: 10.1016/j.gde.2012.09.002. Epub 2012 Oct 11. Curr Opin Genet Dev. 2012. PMID: 23062626 Free PMC article. Review. - The different shades of mammalian pluripotent stem cells.
Kuijk EW, Chuva de Sousa Lopes SM, Geijsen N, Macklon N, Roelen BA. Kuijk EW, et al. Hum Reprod Update. 2011 Mar-Apr;17(2):254-71. doi: 10.1093/humupd/dmq035. Epub 2010 Aug 12. Hum Reprod Update. 2011. PMID: 20705693 Free PMC article. Review.
References
- Mol Cell Biol. 1990 Sep;10(9):4987-9 - PubMed
- Cell. 1976 Sep;9(1):45-55 - PubMed
- Somatic Cell Genet. 1977 May;3(3):247-61 - PubMed
- J Embryol Exp Morphol. 1981 Jun;63:75-84 - PubMed
- Proc Natl Acad Sci U S A. 1982 Jul;79(13):4098-102 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources