Functional analysis of an ascidian homologue of vertebrate Bmp-2/Bmp-4 suggests its role in the inhibition of neural fate specification - PubMed (original) (raw)
Functional analysis of an ascidian homologue of vertebrate Bmp-2/Bmp-4 suggests its role in the inhibition of neural fate specification
T Miya et al. Development. 1997 Dec.
Abstract
The ascidian tadpole larva is thought to be close to a prototype of the ancestral chordate. The vertebrate body plan is established by a series of inductive cellular interactions, whereas ascidians show a highly determinate mode of development. Recent studies however, suggest some roles of cell-cell interaction during ascidian embryogenesis. To elucidate the signaling molecules responsible for the cellular interaction, we isolated HrBMPb, an ascidian homologue of the vertebrate bone morphogenetic protein (BMP) gene, from Halocynthia roretzi. The amino acid sequence of HrBMPb closely resembled those of vertebrate BMP-2 and BMP-4 and of Drosophila Decapentaplegic (DPP). In addition to the sequence similarity, HrBMPb overexpression induced the ventralization of Xenopus embryos, suggesting functional conservation. The zygotic expression of HrBMPb was first detected around gastrulation. HrBMPb expression was maintained in some cells at the lateral edges of the neural plate through gastrulation to neurulation, although that in the presumptive muscle cells was downregulated. HrBMPb was not expressed in the presumptive epidermis during gastrulation. When HrBMPb mRNA was injected into fertilized Halocynthia eggs, cells that normally give rise to the neural tissue differentiated into epidermis, causing a loss of anterior neural tissue in the larva. In addition, HrBMPb might function synergistically with HrBMPa, an ascidian homologue of BMPs-5 to 8. However, HrBMPb overexpression did not affect differentiation of the notochord and muscle cells. These results suggest that HrBMPb functions as a neural inhibitor and as an epidermal inducer but not as a ventralizing agent in ascidian development.
Similar articles
- PCNA mRNA has a 3'UTR antisense to yellow crescent RNA and is localized in ascidian eggs and embryos.
Swalla BJ, Jeffery WR. Swalla BJ, et al. Dev Biol. 1996 Aug 25;178(1):23-34. doi: 10.1006/dbio.1996.0195. Dev Biol. 1996. PMID: 8812106 - The BMP/CHORDIN antagonism controls sensory pigment cell specification and differentiation in the ascidian embryo.
Darras S, Nishida H. Darras S, et al. Dev Biol. 2001 Aug 15;236(2):271-88. doi: 10.1006/dbio.2001.0339. Dev Biol. 2001. PMID: 11476571 - Ascidian embryos as a model system to analyze expression and function of developmental genes.
Satoh N. Satoh N. Differentiation. 2001 Aug;68(1):1-12. doi: 10.1046/j.1432-0436.2001.068001001.x. Differentiation. 2001. PMID: 11683488 Review. - Specification of developmental fates in ascidian embryos: molecular approach to maternal determinants and signaling molecules.
Nishida H. Nishida H. Int Rev Cytol. 2002;217:227-76. doi: 10.1016/s0074-7696(02)17016-1. Int Rev Cytol. 2002. PMID: 12019564 Review.
Cited by
- Brain Sensory Organs of the Ascidian Ciona robusta: Structure, Function and Developmental Mechanisms.
Olivo P, Palladino A, Ristoratore F, Spagnuolo A. Olivo P, et al. Front Cell Dev Biol. 2021 Sep 6;9:701779. doi: 10.3389/fcell.2021.701779. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34552923 Free PMC article. Review. - Control of Pem protein level by localized maternal factors for transcriptional regulation in the germline of the ascidian, Halocynthia roretzi.
Miyaoku K, Nakamoto A, Nishida H, Kumano G. Miyaoku K, et al. PLoS One. 2018 Apr 30;13(4):e0196500. doi: 10.1371/journal.pone.0196500. eCollection 2018. PLoS One. 2018. PMID: 29709000 Free PMC article. - TGF-β Family Signaling in Early Vertebrate Development.
Zinski J, Tajer B, Mullins MC. Zinski J, et al. Cold Spring Harb Perspect Biol. 2018 Jun 1;10(6):a033274. doi: 10.1101/cshperspect.a033274. Cold Spring Harb Perspect Biol. 2018. PMID: 28600394 Free PMC article. Review. - Expression of Hr-Erf Gene during Ascidian Embryogenesis.
Kim JE, Lee WY, Kim GJ. Kim JE, et al. Dev Reprod. 2013 Dec;17(4):389-97. doi: 10.12717/DR.2013.17.4.389. Dev Reprod. 2013. PMID: 25949155 Free PMC article. - The ascidian numb gene involves in the formation of neural tissues.
Ahn HR, Kim GJ. Ahn HR, et al. Dev Reprod. 2012 Dec;16(4):371-8. doi: 10.12717/DR.2012.16.4.371. Dev Reprod. 2012. PMID: 25949112 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources