Phosphodiesterase I, a novel adhesion molecule and/or cytokine involved in oligodendrocyte function - PubMed (original) (raw)

Fig. 3.

Localization of p421.HB/PD-Iα mRNA (A–C, G–I, N–P) in comparison with PLP mRNA (D–F, K–M) in the ventral horn of the spinal cord (A–F), cerebellum (G–M), and choroid plexus of the lateral ventricle (N–P) of postnatal day 4 (A, D, G, K, N) and 21 (B, E, H, L, O) and adult (C, F, I, M, P) rats. p421.HB/PD-Iα mRNA-positive cells were observed in white matter areas in a distribution similar to PLP mRNA-positive cells (compare A with D, B with E, C with F, G with K, and H with L, I with M), although p421.HB/PD-Iα mRNA expression appears to be lower than the one of PLP. In contrast to PLP, at postnatal day 4 no p421.HB/PD-Iα mRNA-positive cells were detectable at the base of the cerebellum (compare G with_K_). In the spinal cord, however, p421. HB/PD-Iα mRNA-positive cells were visible more restricted to the area close to the outer surface (A) than PLP mRNA-positive cells (D). In the adult, only very few p421.HB/PD-Iα mRNA-positive cells were detectable, which were localized in and close to white matter tracts (arrows in_C, I_), where PLP-positive cells can also be detected (compare C with F,I with M). Additional strong p421.HB/PD-Iα mRNA-positive signals were detectable in cells of the choroid plexus, with increasing levels of expression with age (N–P). Sections hybridized with sense cRNA probes showed no labeling. Scale bars (in A): A, B, D, E, 100 μm; C, F, 210 μm; G, H, 110 μm; H, L, 130 μm; I, M, 200 μm; N–P, 20 μm; N–P,insets, 410 μm.