Muscarinic receptor-mediated dual regulation of ADP-ribosyl cyclase in NG108-15 neuronal cell membranes - PubMed (original) (raw)
. 1997 Dec 12;272(50):31272-7.
doi: 10.1074/jbc.272.50.31272.
Affiliations
- PMID: 9395453
- DOI: 10.1074/jbc.272.50.31272
Free article
Muscarinic receptor-mediated dual regulation of ADP-ribosyl cyclase in NG108-15 neuronal cell membranes
H Higashida et al. J Biol Chem. 1997.
Free article
Abstract
Cyclic ADP-ribose (cADP-ribose) is an endogenous modulator of ryanodine-sensitive Ca2+ release channels. An unsolved question is whether or not cADP-ribose mediates intracellular signals from hormone or neurotransmitter receptors. The first step in this study was to develop a TLC method to measure ADP-ribosyl cyclase, by which conversion of [3H]NAD+ to [3H]cADP-ribose was confirmed in COS-7 cells overexpressing human CD38. A membrane fraction of NG108-15 neuroblastoma x glioma hybrid cells possessed ADP-ribosyl cyclase activity measured by TLC. Carbamylcholine increased this activity by 2.6-fold in NG108-15 cells overexpressing m1 or m3 muscarinic acetylcholine receptors (mAChRs), but inhibited it by 30-52% in cells expressing m2 and/or m4 mAChRs. Both of these effects were mimicked by GTP. Pretreatment of cells with cholera toxin blocked the activation, whereas pertussis toxin blocked the inhibition. Application of carbamylcholine caused significant decreases in NAD+ concentrations in untreated m1-transformed NG108-15 cells, but an increase in cholera toxin-treated cells. These results suggest that mAChRs couple to ADP-ribosyl cyclase within cell membranes via trimeric G proteins and can thereby control cellular function by regulating cADP-ribose formation.
Similar articles
- Sympathetic potentiation of cyclic ADP-ribose formation in rat cardiac myocytes.
Higashida H, Egorova A, Higashida C, Zhong ZG, Yokoyama S, Noda M, Zhang JS. Higashida H, et al. J Biol Chem. 1999 Nov 19;274(47):33348-54. doi: 10.1074/jbc.274.47.33348. J Biol Chem. 1999. PMID: 10559213 - Paracrine roles of NAD+ and cyclic ADP-ribose in increasing intracellular calcium and enhancing cell proliferation of 3T3 fibroblasts.
Franco L, Zocchi E, Usai C, Guida L, Bruzzone S, Costa A, De Flora A. Franco L, et al. J Biol Chem. 2001 Jun 15;276(24):21642-8. doi: 10.1074/jbc.M010536200. Epub 2001 Mar 27. J Biol Chem. 2001. PMID: 11274199 - CD38/ADP-ribosyl cyclase: A new role in the regulation of osteoclastic bone resorption.
Sun L, Adebanjo OA, Moonga BS, Corisdeo S, Anandatheerthavarada HK, Biswas G, Arakawa T, Hakeda Y, Koval A, Sodam B, Bevis PJ, Moser AJ, Lai FA, Epstein S, Troen BR, Kumegawa M, Zaidi M. Sun L, et al. J Cell Biol. 1999 Sep 6;146(5):1161-72. doi: 10.1083/jcb.146.5.1161. J Cell Biol. 1999. PMID: 10477767 Free PMC article. - Cyclic ADP-ribose as a second messenger revisited from a new aspect of signal transduction from receptors to ADP-ribosyl cyclase.
Higashida H, Hashii M, Yokoyama S, Hoshi N, Chen XL, Egorova A, Noda M, Zhang JS. Higashida H, et al. Pharmacol Ther. 2001 May-Jun;90(2-3):283-96. doi: 10.1016/s0163-7258(01)00142-5. Pharmacol Ther. 2001. PMID: 11578661 Review. - [Cyclic ADP-ribose as a second messenger--intramembrane signal transduction from receptors to ADP-ribosyl cyclase].
Higashida H, Yokoyama S, Hashii M, Hoshi N. Higashida H, et al. Seikagaku. 1998 Nov;70(11):1326-34. Seikagaku. 1998. PMID: 9889593 Review. Japanese. No abstract available.
Cited by
- CD38-Cyclic ADP-Ribose Signal System in Physiology, Biochemistry, and Pathophysiology.
Takasawa S. Takasawa S. Int J Mol Sci. 2022 Apr 13;23(8):4306. doi: 10.3390/ijms23084306. Int J Mol Sci. 2022. PMID: 35457121 Free PMC article. Review. - Cyclic ADP-ribose as an endogenous inhibitor of the mTOR pathway downstream of dopamine receptors in the mouse striatum.
Higashida H, Kamimura SY, Inoue T, Hori O, Islam MS, Lopatina O, Tsuji C. Higashida H, et al. J Neural Transm (Vienna). 2018 Jan;125(1):17-24. doi: 10.1007/s00702-016-1666-7. Epub 2016 Dec 26. J Neural Transm (Vienna). 2018. PMID: 28025713 - Cyclic ADP-Ribose and NAADP in Vascular Regulation and Diseases.
Li PL, Zhang Y, Abais JM, Ritter JK, Zhang F. Li PL, et al. Messenger (Los Angel). 2013 Jun 1;2(2):63-85. doi: 10.1166/msr.2013.1022. Messenger (Los Angel). 2013. PMID: 24749015 Free PMC article. - Glycosides, depression and suicidal behaviour: the role of glycoside-linked proteins.
Serafini G, Pompili M, Innamorati M, Giordano G, Tatarelli R, Lester D, Girardi P, Dwivedi Y. Serafini G, et al. Molecules. 2011 Mar 23;16(3):2688-713. doi: 10.3390/molecules16032688. Molecules. 2011. PMID: 21441870 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous