The mechanism of Golgi segregation during mitosis is cell type-specific - PubMed (original) (raw)

The mechanism of Golgi segregation during mitosis is cell type-specific

H Stanley et al. Proc Natl Acad Sci U S A. 1997.

Abstract

Golgi membranes in Drosophila embryos and tissue culture cells are found as discrete units dispersed in the cytoplasm. We provide evidence that Golgi membranes do not undergo any dramatic change in their organization during the rapid mitotic divisions of the nuclei in the syncitial embryo or during cell division postcellularization. By contrast, in Drosophila tissue culture cells, the Golgi membranes undergo complete fragmentation during mitosis. Our studies show that the mechanism of Golgi partitioning during cell division is cell type-specific.

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Figures

Figure 1

Figure 1

Organization of Golgi membranes in precellularized embryos. (A) Nonmitotic nuclei. (B) Metaphase nuclei. (C) Late anaphase nuclei. The Golgi membranes were stained with a Golgi-specific antibody that recognizes a 120-kDa integral Golgi membrane protein. DNA was stained with propidium iodide. The Golgi membranes appear randomly dispersed around the nuclear material, and the organization remains unchanged during the mitotic cycle. (Bars = 10 μ.)

Figure 2

Figure 2

The organization of Golgi membranes in cellularized embryos. The mitotic cells are shown by arrowheads in A–D. B and D are higher magnifications of A and C, respectively. The distribution of Golgi membranes in mitotic and nonmitotic cells appears similar by immunofluorescence microscopy by using Golgi monoclonal antibody. The DNA is stained with propidium iodide.

Figure 3

Figure 3

The organization of Golgi membranes in Drosophila tissue culture cells. (A) In interphase cells the Golgi membranes appear as discrete units dispersed in the cytoplasm. In mitotic cells (marked with arrowheads) the Golgi membranes appear completely fragmented and diffusely dispersed in the cytoplasm. (B) The nuclear envelop stained with a specific monoclonal antibody appears fragmented in mitotic cells (arrowhead). The DNA was stained with propidium iodide. Thus, both the Golgi membranes and the nuclear envelope break down during mitosis in the Drosophila tissue culture cells.

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