FGF-2 antisense RNA encodes a nuclear protein with MutT-like antimutator activity - PubMed (original) (raw)

FGF-2 antisense RNA encodes a nuclear protein with MutT-like antimutator activity

A W Li et al. Mol Cell Endocrinol. 1997.

Abstract

Bidirectional transcription of the basic fibroblast growth factor (FGF-2) gene gives rise to multiple polyadenylated sense mRNAs and a unique 1.5 kb antisense transcript (FGF-AS) which is complementary to the 3'-untranslated region of the FGF-2 mRNA. The rat FGF-AS cDNA encodes a novel 35 kDa nuclear protein (GFG) with homology to the MutT family of antimutator NTPases. Antibodies against the deduced amino acid sequence of GFG detected intense immunoreactivity in the nuclei of adult rat hepatocytes. Subcellular fractionation and Western blotting confirmed the presence of a 35 kDa immunoreactive protein in the nuclear fraction and, to a lesser extent, in the mitochondrial fractions of rat liver homogenates. Recombinant GFG suppressed the spontaneous mutation rate of MutT-deficient E. coli in a complementation assay. In-frame deletion of the 53 amino acids encompassing the MutT domain eliminated this activity, confirming the catalytic function of this region in the FGF antisense gene product. These findings demonstrate for the first time that the FGF-AS transcript encodes a functional nuclear protein with MutT-related enzymatic activity.

PubMed Disclaimer

Similar articles

• The basic fibroblast growth factor (FGF-2) antisense RNA (GFG) is translated into a MutT-related protein in vivo.
  Li AW, Too CK, Murphy PR. Li AW, et al. Biochem Biophys Res Commun. 1996 Jun 5;223(1):19-23. doi: 10.1006/bbrc.1996.0839. Biochem Biophys Res Commun. 1996. PMID: 8660369
• The endogenous fibroblast growth factor-2 antisense gene product regulates pituitary cell growth and hormone production.
  Asa SL, Ramyar L, Murphy PR, Li AW, Ezzat S. Asa SL, et al. Mol Endocrinol. 2001 Apr;15(4):589-99. doi: 10.1210/mend.15.4.0626. Mol Endocrinol. 2001. PMID: 11266510
• Characterization of the mutX gene of Streptococcus pneumoniae as a homologue of Escherichia coli mutT, and tentative definition of a catalytic domain of the dGTP pyrophosphohydrolases.
  Méjean V, Salles C, Bullions LC, Bessman MJ, Claverys JP. Méjean V, et al. Mol Microbiol. 1994 Jan;11(2):323-30. doi: 10.1111/j.1365-2958.1994.tb00312.x. Mol Microbiol. 1994. PMID: 8170394
• The MutT motif family of nucleotide phosphohydrolases in man and human pathogens (review).
  McLennan AG. McLennan AG. Int J Mol Med. 1999 Jul;4(1):79-89. doi: 10.3892/ijmm.4.1.79. Int J Mol Med. 1999. PMID: 10373642 Review.
• The role of the mutT gene of Escherichia coli in maintaining replication fidelity.
  Fowler RG, Schaaper RM. Fowler RG, et al. FEMS Microbiol Rev. 1997 Aug;21(1):43-54. doi: 10.1111/j.1574-6976.1997.tb00344.x. FEMS Microbiol Rev. 1997. PMID: 9299701 Review.

Cited by

• A comprehensive structural, biochemical and biological profiling of the human NUDIX hydrolase family.
  Carreras-Puigvert J, Zitnik M, Jemth AS, Carter M, Unterlass JE, Hallström B, Loseva O, Karem Z, Calderón-Montaño JM, Lindskog C, Edqvist PH, Matuszewski DJ, Ait Blal H, Berntsson RPA, Häggblad M, Martens U, Studham M, Lundgren B, Wählby C, Sonnhammer ELL, Lundberg E, Stenmark P, Zupan B, Helleday T. Carreras-Puigvert J, et al. Nat Commun. 2017 Nov 16;8(1):1541. doi: 10.1038/s41467-017-01642-w. Nat Commun. 2017. PMID: 29142246 Free PMC article.
• Substrate ambiguity among the nudix hydrolases: biologically significant, evolutionary remnant, or both?
  McLennan AG. McLennan AG. Cell Mol Life Sci. 2013 Feb;70(3):373-85. doi: 10.1007/s00018-012-1210-3. Epub 2012 Nov 27. Cell Mol Life Sci. 2013. PMID: 23184251 Free PMC article.
• Endogenous retroviral pathogenesis in lupus.
  Perl A, Fernandez D, Telarico T, Phillips PE. Perl A, et al. Curr Opin Rheumatol. 2010 Sep;22(5):483-92. doi: 10.1097/BOR.0b013e32833c6297. Curr Opin Rheumatol. 2010. PMID: 20644481 Free PMC article. Review.
• Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.
  MacFarlane LA, Gu Y, Casson AG, Murphy PR. MacFarlane LA, et al. Mol Endocrinol. 2010 Apr;24(4):800-12. doi: 10.1210/me.2009-0367. Epub 2010 Mar 2. Mol Endocrinol. 2010. PMID: 20197313 Free PMC article.
• Overlapping antisense transcription in the human genome.
  Fahey ME, Moore TF, Higgins DG. Fahey ME, et al. Comp Funct Genomics. 2002;3(3):244-53. doi: 10.1002/cfg.173. Comp Funct Genomics. 2002. PMID: 18628857 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Molecular Biology Databases

  • GlyGen glycoinformatics resource

• Research Materials

  • NCI CPTC Antibody Characterization Program